Analysis of Phosphatidylinositol Transfer at ER-PM Junctions in Receptor-Stimulated Live Cells.
Analysis of Phosphatidylinositol Transfer at ER-PM Junctions in Receptor-Stimulated Live Cells. Methods Mol Biol. 2019;1949:1-11 Authors: Chang CL, Liou J Abstract Phosphatidylinositol (PI) is an inositol-containing phospholipid synthesized in the endoplasmic reticulum (ER). PI is a precursor lipid for PI 4,5-bisphosphate (PI(4,5)P2) in the plasma membrane (PM) important for Ca2+ signaling in response to extracellular stimuli. Thus, ER-to-PM PI transfer becomes essential for cells to maintain PI(4,5)P2 homeostasis during receptor stimulation. In this chapter, we discuss two live-cell imaging protocols to analyze ER-to-PM PI transfer at ER-PM junctions, where the two membrane compartments make close appositions accommodating PI transfer. First, we describe how to monitor PI(4,5)P2 replenishment following receptor stimulation, as a readout of PI transfer, using a PI(4,5)P2 biosensor and total internal reflection fluorescence microscopy. The second protocol directly visualizes PI transfer proteins that accumulate at ER-PM junctions and mediate PI(4,5)P2 replenishment with PI in the ER in stimulated cells. These methods provide spatial and temporal analysis of ER-to-PM PI transfer during receptor stimulation and can be adapted to other research questions related to this topic. PMID: 30790244 [PubMed - indexed for MEDLINE]
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Review of "Atlas of Artifacts in Clinical Neurophysiology," by William O. Tatum, IV Atlas of Artifacts in Clinical Neurophysiology by William O. Tatum , IV, Demos Medical Publishing, 2018 , 419 pages. ISBN: 9780826169341 . E-book ISBN: 978-0826169358 . List Price: $185.00; Amazon New: $154.02; Amazon Used: $144.00; Kindle: $129.36. Neurodiagn J. 2019 Aug 23;:1-2 Authors: Clark-Bash RJ PMID: 31441708 [PubMed - as supplied by publisher]
Authors: Moosavian SP, Arab A, Mehrabani S, Moradi S, Nasirian M Abstract Background: Several studies have investigated the effect of omega-3 fatty acids and vitamin E on oxidative stress and inflammation, but their findings are inconsistent. The aim of this meta-analysis is to elucidate the overall effects of co-supplementation with omega-3 fatty acids and vitamin E on oxidative stress and inflammation. Methods: We searched titles, abstracts, and keywords of relevant articles indexed in PubMed, ISI, Scopus, and Google Scholar databases up to December 2018 to identify eligible RCT studies. Random effects model was...
Conclusion: These results provide a potential mechanism involving AMPK activation/ROS inhibition signaling pathway in osteogenic differentiation of BMSCs enhanced by resveratrol. It suggests that development of therapy towards ROS is an effective way for osteoporosis treatment. PMID: 31438780 [PubMed - in process]
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