Measurement of Lysophospholipid Transport Across the Membrane Using Escherichia coli Spheroplasts.

Measurement of Lysophospholipid Transport Across the Membrane Using Escherichia coli Spheroplasts. Methods Mol Biol. 2019;1949:165-180 Authors: Lin Y, Zheng L, Bogdanov M Abstract In the inner membrane of Gram-negative bacteria lysophospholipid transporter (LplT) and the bifunctional acyl-acyl carrier protein (ACP) synthetase/2-acylglycerolphosphoethanolamine acyltransferase (Aas) form a glycerophospholipid remodeling system, which is capable of facilitating rapid retrograde translocation of lyso forms of phosphatidylethanolamine, phosphatidylglycerol, and cardiolipin across the cytoplasmic membrane. This coupled remodeling enzyme tandem provides an effective method for the measurement of substrate specificity of the lipid regeneration and lysophospholipid transport per se across the membrane. This chapter describes two distinct but complementary methods for the measurement of lysophospholipid transport across membrane using Escherichia coli spheroplasts. PMID: 30790256 [PubMed - indexed for MEDLINE]
Source: Mol Biol Cell - Category: Molecular Biology Authors: Tags: Methods Mol Biol Source Type: research

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ConclusionsThis study revealed a high prevalence of rectal CPE colonization in high-risk patients from ICU and HSCT wards, and a predominant colonization of the KPC-producingK. pneumoniae clone ST11. Stricter infection control measures are urgently needed to limit the dissemination of CPE strains, especially in patients who were afflicted by urinary system diseases, have underwent bronchoscopy, and were previously exposed to combined antibiotic use.
Source: Antimicrobial Resistance and Infection Control - Category: Infectious Diseases Source Type: research
In this study, surface ‐enhanced infrared absorption spectroscopy applied to the immobilized LacY was used to study the pH‐dependent changes in LacY and to accessin  situ the effect of the 4B1 antibody on the pKa of Glu325, the primary functional H+‐binding site in LacY. A small shift of the pK value from 10.5 to 9.5 was identified that can be corroborated with the inactivation of LacY upon 4B1 binding.
Source: FEBS Letters - Category: Biochemistry Authors: Tags: Research Letter Source Type: research
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Source: Medical Science Monitor - Category: Research Tags: Med Sci Monit Source Type: research
In this study, surface ‐enhanced infrared absorption spectroscopy (SEIRAS) applied to the immobilized LacY was used to study the pH‐dependent changes in LacY and to access in situ the effect of the 4B1 antibody on the pKa of Glu325, the primary functional H+‐binding site in LacY. A small shift of the pK value from 10.5 to 9.5 was identified that can be corroborated with the inactivation of LacY upon 4B1 binding.
Source: FEBS Letters - Category: Biochemistry Authors: Tags: RESEARCH LETTER Source Type: research
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Source: Applied Microbiology and Biotechnology - Category: Microbiology Authors: Tags: Appl Microbiol Biotechnol Source Type: research
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Source: Frontiers in Immunology - Category: Allergy & Immunology Source Type: research
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Source: Infection and Immunity - Category: Infectious Diseases Authors: Tags: Host Response and Inflammation Source Type: research
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Source: Journal of Biological Chemistry - Category: Chemistry Authors: Tags: Gene Regulation Source Type: research
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Source: PLoS Biology: Archived Table of Contents - Category: Biology Authors: Source Type: research
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