Cancers, Vol. 11, Pages 990: Anti-tumor Activity and Epigenetic Impact of the Polyphenol Oleacein in Multiple Myeloma

Cancers, Vol. 11, Pages 990: Anti-tumor Activity and Epigenetic Impact of the Polyphenol Oleacein in Multiple Myeloma Cancers doi: 10.3390/cancers11070990 Authors: Giada Juli Manuela Oliverio Dina Bellizzi Maria Eugenia Gallo Cantafio Katia Grillone Giuseppe Passarino Carmela Colica Monica Nardi Marco Rossi Antonio Procopio Pierosandro Tagliaferri Pierfrancesco Tassone Nicola Amodio Olive oil contains different biologically active polyphenols, among which oleacein, the most abundant secoiridoid, has recently emerged for its beneficial properties in various disease contexts. By using in vitro models of human multiple myeloma (MM), we here investigated the anti-tumor potential of oleacein and the underlying bio-molecular sequelae. Within a low micromolar range, oleacein reduced the viability of MM primary samples and cell lines even in the presence of bone marrow stromal cells (BMSCs), while sparing healthy peripheral blood mononuclear cells. We also demonstrated that oleacein inhibited MM cell clonogenicity, prompted cell cycle blockade and triggered apoptosis. We evaluated the epigenetic impact of oleacein on MM cells, and observed dose-dependent accumulation of both acetylated histones and α-tubulin, along with down-regulation of several class I/II histone deacetylases (HDACs) both at the mRNA and protein level, providing evidence of the HDAC inhibitory activity of this compound; conversely, no effect on global DNA methylation wa...
Source: Cancers - Category: Cancer & Oncology Authors: Tags: Article Source Type: research