Molecular mechanisms for intestinal HCO3- secretion and its regulation by guanylin in seawater-acclimated eels RESEARCH ARTICLE

Yoshio Takei, Marty K. S. Wong, and Masaaki Ando The intestine of marine teleosts secretes HCO3– into the lumen and precipitates Ca2+ and Mg2+ in the imbibed seawater as carbonates to decrease luminal fluid osmolality and facilitate water absorption. However, the hormonal regulation of HCO3– secretion is largely unknown. Here, mucosally added guanylin (GN) increased HCO3– secretion, measured by pH-stat, across isolated seawater-acclimated eel intestine bathed in saline at pH 7.4 (5% CO2). The effect of GN on HCO3– secretion was slower than that on the short-circuit current, and the time course of the GN effect was similar to that of bumetanide. Mucosal bumetanide and serosal 4,4'-dinitrostilbene-2,2'-disulfonic acid (DNDS) inhibited the GN effect, suggesting an involvement of apical Na+-K+-2Cl– cotransporter (NKCC2) and basolateral Cl–/HCO3– exchanger (AE)/Na+-HCO3– cotransporter (NBC) in the GN effect. As mucosal DNDS failed to inhibit the GN effect, apical DNDS-sensitive AE may not be involved. To identify molecular species of transporters involved in the GN effect, we performed RNA-seq analyses followed by quantitative real-time PCR after transfer of eels to seawater. Among the genes upregulated after seawater transfer, AE genes (draa, b, and pat1a, c) on the apical membrane, and NBC genes (nbce1a, n1, n2a) and an AE gene (sat-1) on the basolateral membrane were candidates involved in HCO3– secretion. Judging from...
Source: Journal of Experimental Biology - Category: Biology Authors: Tags: RESEARCH ARTICLE Source Type: research

Related Links:

Publication date: Available online 24 August 2019Source: Sport Management ReviewAuthor(s): Karolina Eszter Kovács
Source: Sport Management Review - Category: Sports Medicine Source Type: research
Publication date: Available online 23 August 2019Source: Journal of Vascular and Interventional RadiologyAuthor(s): Bahman Roudsari, Rex Pillai, Catherine Vu
Source: Journal of Vascular and Interventional Radiology - Category: Radiology Source Type: research
We examined whether advanced MRI analysis techniques have the capacity to detect brain injury (particularly white matter injury) caused by chronic hypoxia-induced fetal growth restriction in newborn preterm lambs.MethodsSurgery was undertaken in twin bearing pregnant ewes at 88–90 days gestation (term = 150 days) to induce FGR in one fetus. At 127 days gestation (~32 weeks human brain development), FGR and control (appropriate for gestational age, AGA) lambs were delivered by caesarean section, intubated and ventilated. Conventional and advanced brain imaging was conducted within the first two hours of li...
Source: NeuroImage: Clinical - Category: Radiology Source Type: research
Publication date: September 2019Source: Diagnostic and Interventional Imaging, Volume 100, Issue 9Author(s):
Source: Diagnostic and Interventional Imaging - Category: Radiology Source Type: research
Publication date: September 2019Source: Diagnostic and Interventional Imaging, Volume 100, Issue 9Author(s):
Source: Diagnostic and Interventional Imaging - Category: Radiology Source Type: research
Publication date: Available online 23 August 2019Source: The Social Science JournalAuthor(s): Zefeng BaiAbstractThe present study investigates the impact of two drinking habits – moderate drinking and heavy drinking – on marijuana use among college-aged youths. Utilizing data from the National Longitudinal Survey of Youth 97 (NLSY97), this paper reveals that there is a positive association between both drinking habits and marijuana use in the long run, indicating that alcohol and marijuana are complements. However, in the short run, the association between marijuana and alcohol varies based on different drinkin...
Source: The Social Science Journal - Category: Psychiatry & Psychology Source Type: research
Publication date: Available online 23 August 2019Source: Social Science &MedicineAuthor(s): Megan D. CogburnAbstractOver the last two decades, there has been a global push to improve maternal health by increasing numbers of facility births in low- and middle-income countries like Tanzania. While recent scholarship has interrogated the increasing hegemony of numbers and metrics in global health, few have ethnographically explored how this push for numbers and its accompanying technologies affect the lived experiences of parturients and those who care for them during pregnancy and childbirth in rural communities. Based o...
Source: Social Science and Medicine - Category: Psychiatry & Psychology Source Type: research
This study investigated the association of the five-factor model and trait emotional intelligence (trait EI) with camouflaging behaviours (masking and compensating for autistic characteristics) in a large sample of 278 autistic and 230 typically developing (TD) participants. Participants completed questionnaires assessing autistic traits, big five personality, trait EI, and social camouflaging of autistic traits. Camouflaging was negatively related to extraversion, agreeableness, and conscientiousness, while it was positively related to neuroticism. Trait EI was also negatively linked to camouflaging. The differences betwe...
Source: Personality and Individual Differences - Category: Psychiatry & Psychology Source Type: research
We examined consequences of work-family guilt with an interview study (N = 28) and daily diary study (N = 123). The interview study revealed that as a result of work-family guilt, parents tended to either reappraise the situation (e.g., emphasizing financial importance of work) or compensate for their guilt by adapting their parenting, adapting their work, and by sacrificing their leisure. Consistently, the diary study (where mothers completed online daily questionnaires over 8 consecutive days) revealed that higher work-family guilt was related to more traditional gender behaviors in mothers. Specifically, mothers...
Source: Journal of Vocational Behavior - Category: Psychiatry & Psychology Source Type: research
Conclusions: The new instrument allows healthcare professionals to assess the level of person-centered care provided by gerontology centers with excellent reliability and validity.ResumenAntecedentes/Objetivo: Las residencias y otros servicios gerontológicos son frecuentemente criticados por su falta de flexibilización y personalización en la atención, conduciendo a un trato uniforme que dificulta el control sobre sus vidas. El objetivo del trabajo es desarrollar y validar el primer instrumento de medida español que permite a los profesionales de la salud evaluar en qué medida se l...
Source: International Journal of Clinical and Health Psychology - Category: Psychiatry & Psychology Source Type: research
More News: Biology | Bumetanide | Genetics | Hormones | Molecular Biology