Molecular phylodiagnosis of Echinococcus granulosus sensu lato and Taenia hydatigena determined by mitochondrial Cox1 and SSU-rDNA markers in Iranian dogs: Indicating the first record of pig strain (G7) in definitive host in the Middle East.

Molecular phylodiagnosis of Echinococcus granulosus sensu lato and Taenia hydatigena determined by mitochondrial Cox1 and SSU-rDNA markers in Iranian dogs: Indicating the first record of pig strain (G7) in definitive host in the Middle East. Comp Immunol Microbiol Infect Dis. 2019 Aug;65:88-95 Authors: Mirbadie SR, Najafi Nasab A, Mohaghegh MA, Norouzi P, Mirzaii M, Spotin A Abstract Unawareness of canine parasitic diseases among at-risk hosts and an uncontrolled program of stray dog population have caused that zoonotic parasites received great attention in endemic regions of the Middle East. A total of 552 faecal samples were collected between December 2016 to January 2018 from stray (n = 408) and domestic (n = 144) dogs of Iran. All specimens were coproscopically observed following concentration and flotation techniques. Subsequently, the DNAs of taeniid eggs were extracted, amplified, and sequenced by targeting of mitochondrial cytochrome oxidase subunit 1 and small-subunit ribosomal DNA markers. The overall prevalence of canine intestinal parasites found 53.6%. The following parasites and their total frequencies were identified: taeniid (10.5%), Dicrocoelium dendriticum (0.7%), Trichuris vulpis (1.2%), Capillaria spp. (2.3%), Blastocystis spp. (5.2%), Ancylostoma spp. (2%), Eimeria spp. (13.2%), Dipylidium caninum (2.3%), Toxocara canis (3.8%), Giardia spp. (8.5%), and Toxascaris leonina (3.6%). Stray dogs were characteriz...
Source: Comparative immunology, microbiology and infectious diseases. - Category: Allergy & Immunology Authors: Tags: Comp Immunol Microbiol Infect Dis Source Type: research

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CONCLUSION: Ultrastructural changes in rat liver tissue in conditions of obstructive jaundice may be reversible after restoration of drainage. On the other hand, ultrastructural changes in renal tissue in cases of prolonged jaundice are irreversible even if the internal drainage is restored. KEY WORDS: Bile Duct, Liver, Kidney, Obstructive Jaundice. PMID: 31630139 [PubMed - as supplied by publisher]
Source: Annali Italiani di Chirurgia - Category: Surgery Tags: Ann Ital Chir Source Type: research
Publication date: Available online 20 October 2019Source: Protein Expression and PurificationAuthor(s): Lei Jing, Juanjuan Liu, Dongxu Cui, Yuyin Li, Zhenxing Liu, Li Tao, Qing Zhao, Aipo DiaoAbstractAn affibody is a 58 amino acids peptide derived from the Z domain of staphylococcal protein A and generally applied in areas such as imaging diagnosis, clinical therapeutics and biotechnology research. To screen for an affibody targeting the immune checkpoint PD-L1, a combinatorial affibody library was generated in yeast using degenerate overlap PCR primers and In-fusion technology. Z-j1 and Z-j2 affibodies targeting the Ig-li...
Source: Protein Expression and Purification - Category: Biochemistry Source Type: research
Publication date: Available online 20 October 2019Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Tomasz Janek, Lígia R. Rodrigues, Eduardo J. Gudiña, Joanna BurgerAbstractThe interactions between two types of quaternary ammonium surfactants (N,N,N-trimethyl-2-(dodecanoyloxy)ethaneammonium bromide (DMM-11) and N,N,N-trimethyl-2-(dodecanoyloxy)propaneammonium bromide (DMPM-11)) and hen egg white lysozyme were studied through several techniques, including isothermal titration calorimetry (ITC), circular dichroism (CD) and fluorescence spectroscopy, and surface tension measurement. The average number of...
Source: Colloids and Surfaces B: Biointerfaces - Category: Biochemistry Source Type: research
Publication date: January 2020Source: Phytochemistry, Volume 169Author(s): Behnaz Tohidi, Mehdi Rahimmalek, Ahmad Arzani, Helena TrindadeAbstractThyme (Thymus spp.) is a valuable genus of Lamiaceae family with different pharmaceutical and food properties. Thymol has also been considered as the major essential oil compound in most of the studied Thymus species. In this research, the gene encoding γ-terpinene synthase (Ttps2) was sequenced in T. vulgaris and in eight Iranian thymes including T. carmanicus, T. daenensis, T. fedtschenkoi, T. kotschyanus, T. migricus, T. pubescens, T. se...
Source: Phytochemistry - Category: Chemistry Source Type: research
In this study, a chemometric strategy was developed for analysis of gas chromatographic (GC) and infrared spectroscopic (FT-IR) fingerprints of nine crude oil samples from the main oil wells of Iran to classify them and to find their origins. In this regard, a fractionation method based on saturated, aromatic, resin, and asphaltene (SARA) test was used. Then, these fractions were analyzed by GC-FID and GC-MS. Also, nine crude oil samples were analyzed by FT-IR.The obtained GC fingerprints were aligned using correlation optimized warping (COW) and auto-scaled, and then analyzed using principal component analysis (PCA) and h...
Source: Microchemical Journal - Category: Chemistry Source Type: research
Phosphoenolpyruvate carboxykinase (PEPCK) is a ubiquitous enzyme found in all known groups of organisms, acting in the reversible conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP) in the presence of divalent metal ion, and dependent of adenosine 5 ’ -triphosphate (ATP) or guanosine-5 ’ -triphosphate (GTP). PEPCK is an important enzyme in the metabolism of some organisms, such as Trypanosoma cruzi, being suggested as a potential drug target to treat Chagas ’ disease. Its catalytic activity is, classically, measured by coupled assays. Herein, a direct assay by liquid chromatography-tandem mass s...
Source: Journal of the Brazilian Chemical Society - Category: Chemistry Source Type: research
Conclusions: Naturally acquired sexual stage immunity, as detected by antibodies to Pfs230 and Pfs48/45, was present in most studies analyzed. Significant between-study heterogeneity was seen, and methodological factors were a major contributor to this, and prevented further analysis of epidemiological and biological factors. This demonstrates a need for standardized protocols for conducting and reporting seroepidemiological analyses.
Source: Frontiers in Immunology - Category: Allergy & Immunology Source Type: research
This study explored the association between serum vitamin D levels and urinary tract infections in pregnant women.Study designIn this case control study, 187 participants including, 97 pregnant women diagnosed as a symptomatic UTI (case group) and 90 matched healthy pregnant women (control group) were consecutively enrolled from prenatal care clinic of Imam Reza Hospital in Urmia, North West of Iran. The two groups were matched for trimester and parity, and sexual intercourse. Blood samples were collected from both groups. Chemiluminescent immunoassay (CLIA) was used to evaluate the serum vitamin D levels. We used a binary...
Source: European Journal of Obstetrics and Gynecology and Reproductive Biology - Category: OBGYN Source Type: research
Publication date: December 2019Source: Molecular Immunology, Volume 116Author(s): Bijara Devi Sanasam, Sanjeev KumarAbstractMalaria caused by Plasmodium falciparum is the most deadly and a major health issue worldwide. In spite of several control programs, there hasn’t been much improvement in keeping the disease under control. The appearance of drug resistant strains of Plasmodium in addition to insecticide resistance of the Anopheles vector has been a hurdle. Therefore, it is highly desirable to identify new potential candidates that can be targeted for therapeutic intervention. The present study identifies AMR1, a...
Source: Molecular Immunology - Category: Allergy & Immunology Source Type: research
This week's case was generously donated by Professor Agnes Kurniawan from the University of Indonesia. The following motile structure was reported to emerge from the anus of a man from rural Indonesia. He had no other gastrointestinal symptoms.You can see its subtle movement here:This structure was expressed from the submitted object:Identification?
Source: Creepy Dreadful Wonderful Parasites - Category: Parasitology Source Type: blogs
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