An optimized yeast display strategy for efficient scFv antibody selection using ribosomal skipping system and thermo resistant yeast.
CONCLUSIONS: The method highlights the utility of the T2A-Leu2-based ribosomal skipping strategy for increasing the quality of the input library for selection, along with an optimized selection protocol based on thermo-resistant yeast cells.
PMID: 31300936 [PubMed - as supplied by publisher]
Source: Biotechnology Letters - Category: Biotechnology Authors: Jia Y, Ren P, Duan S, Zeng P, Xie D, Zeng F Tags: Biotechnol Lett Source Type: research
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