Generation of 5 induced pluripotent stem cell lines, LUMCi007-A and B and LUMCi008-A, B and C, from 2 patients with Huntington disease

Publication date: Available online 12 July 2019Source: Stem Cell ResearchAuthor(s): Linda M. van der Graaf, Sarah L. Gardiner, Merve Tok, Tom Brands, Merel W. Boogaard, Barry A. Pepers, Bert Eussen, Annelies de Klein, N. Ahmad Aziz, Christian Freund, Ronald A.M. Buijsen, Willeke M.C. van Roon-MomAbstractHuntington disease (HD) is an autosomal dominant, neurodegenerative disease caused by a CAG repeat expansion within the coding sequence of the HTT gene, resulting in a highly toxic protein with an expanded polyglutamine stretch that forms typical protein aggregates throughout the brain. We generated human induced pluripotent stem cells (hiPSCs) from two HD patients using non-integrating Sendai virus (SeV). The hiPSCs display a normal karyotype, express all pluripotency markers, have the same CAG repeat expansion as the original fibroblasts and are able to differentiate into the three germ layers in vitro.
Source: Stem Cell Research - Category: Stem Cells Source Type: research