Potent anti-MRSA activity and synergism with aminoglycosides by flavonoid derivatives from the root barks of Morus alba , a traditional Chinese medicine

AbstractInfections of clinical methicillin-resistantStaphylococcus aureus (MRSA) is a very tough public health problem and a challenge of new drug development. Nearly 90 Diels-Alder adducts (DAAs) have so far been isolated fromMorus plants, but only a few of them have been evaluated for their anti-MRSA activities. To study the antibacterial compounds of DAAs from the root barks ’ section ofMorus alba L. and their synergism with antibacterial agents against clinical MRSA strains, bioassay-guided phytochemical methods were used to screen the active components. Minimal inhibitory concentrations (MICs) and minimal bactericidal concentrations (MBCs) were assayed through broth serial microdilution. The synergism were evaluated by checker board microdilution and dynamic time-kill experiments. Three DAAs (multicaulisin (1), sanggenon G (2) and albanin G (3)) were isolated and identified fromM. alba root barks. They were determined with potent effect against MRSA isolates with MICs/MBCs at 2 –8/16–128 mg/L. They also showed synergy with conventional antibacterial agents, especially the aminoglycosides, with fractional inhibitory concentration indices (FICIs) ranged from 0.19 to 0.50 and the dose reduction indices (DRIs) ranged from 16 to 2. The MRSA resistance to the antibiotics c ould be reversed by compounds1–3. The dose-dependent bactericidal synergism against MRSA was observed as well. The study released for the first time the anti-MRSA synergism of ...
Source: Medicinal Chemistry Research - Category: Chemistry Source Type: research

Related Links:

The objective of this study was to evaluate hybrid meshes (Gore® Synecor, Zenapro™, Ovitex™ 1S Reinforced Bioscaffold Permanent, and Ovitex™ 1S Reinforced Bioscaffold Resorbable) compared to non-hybrid, bioresorbable synthetic mesh (Phasix™ Mesh) in a rabbit bacterial inoculation model.Materials and methodsSubcutaneous pockets were bilaterally created in male, New Zealand White rabbits (n = 25). Circular meshes (3.8 cm diameter) were implanted and inoculated with 1 × 106 colony forming units (CFU) of clinically-isolated methicillin-resistant Staphylococcus aureus (MRSA). A given ...
Source: Annals of Medicine and Surgery - Category: General Medicine Source Type: research
ConclusionOverall our results show a global increase in the prevalence of HLMuRSA and HLMuRMRSA among clinical S. aureus isolates over time. However, there was only a significant increase in the prevalence of MuRMRSA compared to the other categories of strains especially MuRSA. Since for decolonization of MSSA and MRSA in both patients and healthcare personnel, mupirocin is currently still the most effective antibiotic, a reduction of its effectiveness can provide a risk for invasive infection. Monitoring of mupirocin resistance development remains critical.
Source: Journal of Global Antimicrobial Resistance - Category: Infectious Diseases Source Type: research
Background: Cellulitis is an infection of the skin and subcutaneous tissue presenting with erythema, swelling, warmth, and pain. It is a common cause of skin and soft tissue infections, resulting in 650,000 hospital admissions per year in the United States alone. Antibiotic choice during hospitalization and on discharge is complicated by increasing concern about antibiotic-resistant bacteria, resulting in use of anti-MRSA antibiotics or broad-spectrum antibiotics. The Infectious Diseases Society of America has published guidelines for management of cellulitis, though whether these guidelines are implemented in everyday practice is unknown.
Source: Journal of the American Academy of Dermatology - Category: Dermatology Source Type: research
Background: Ozenoxacin (OZN), a novel nonfluorinated quinolone for the topical treatment of impetigo has shown to be effective against MRSA isolates.
Source: Journal of the American Academy of Dermatology - Category: Dermatology Source Type: research
On 9 August 2019,  Betty Carnegie “Monty” Montgomery, aged 82 years, of Glasgow. A former member of the Royal Pharmaceutical Society, Mrs Montgomery registered with the Society in 1960 and was designated Fellow in 1983. She was elected to the Royal Pharmaceutical Society of Great Britain’s Scottish Executive in 1975 and chaired the Scottish Executive from 1984 until 1986. 
Source: The Pharmaceutical Journal - Category: Drugs & Pharmacology Source Type: research
In conclusion, the extracts of Auricularia spp. and Termitomyces spp. have shown promising antimicrobial and antioxidant activities. PMID: 31427902 [PubMed - in process]
Source: The Scientific World Journal - Category: Science Tags: ScientificWorldJournal Source Type: research
Abstract Recently, there has been an increased tendency towards raw-milk consumption, which may pose a consumer risk, due to the possible presence of human pathogenic microorganisms, such as Staphylococcus aureus and even methicillin-resistant S. aureus (MRSA). The prevalence of S. aureus and methicillin-resistant S. aureus (MRSA) was investigated in 40 dairy (cattle, sheep and goat) farms in northern Greece. S. aureus and MRSA were detected in 47.8% and 4.1% of the 387 samples (raw milk, farmers and animal samples) tested, respectively. Most (81.3%) of the MRSA isolates harbored the mecA gene, whereas the mecC or...
Source: Food Microbiology - Category: Food Science Authors: Tags: Food Microbiol Source Type: research
Source: Infection and Drug Resistance - Category: Infectious Diseases Tags: Infection and Drug Resistance Source Type: research
Conclusion: This present findings of multi-drug resistance MRSA is very worrisome as it further highlights the pressing need  to keep a strict watch on MRSA emerging from this study area.Keywords: MRSA, beta-lactamase, antibiotics, multidrug resistance, clinical samples, community samples.
Source: African Health Sciences - Category: African Health Source Type: research
Halogenated boroxine dipotassium trioxohydroxytetrafluorotriborate K2[B3O3F4OH] inhibits emerging multidrug-resistant and β-lactamase-producing opportunistic pathogens. Drug Dev Ind Pharm. 2019 Aug 16;:1-21 Authors: Maravić A, Rončević T, Krce L, Ilić N, Galić B, Čikeš Čulić V, Carev I Abstract Halogenated boroxine dipotassium trioxohydroxytetrafluorotriborate, K2[B3O3F4OH] (boroxine) was previously shown to be very effective in inhibition of several carcinoma cell lines, including the skin cancer. Here, we investigated its antimicrobial potential by targeting the multidrug-resistan...
Source: Drug Development and Industrial Pharmacy - Category: Drugs & Pharmacology Tags: Drug Dev Ind Pharm Source Type: research
More News: Chemistry | China Health | Flavonoids | Infectious Diseases | International Medicine & Public Health | MRSA | Study | Superbugs