Achieving cytochrome c fibril/aggregate control towards micro-platelets and micro-fibers by tuning pH and protein concentration: A combined morphological and spectroscopic analysis.

Achieving cytochrome c fibril/aggregate control towards micro-platelets and micro-fibers by tuning pH and protein concentration: A combined morphological and spectroscopic analysis. Int J Biol Macromol. 2019 Jul 08;: Authors: Nucara A, Carbone M, Ripanti F, Manganiello R, Postorino P, Carbonaro M Abstract Cytochrome c is a metalloprotein with primary physiological functions in the respiratory chain and in the regulation of cell death signals. Investigating the mechanisms leading to cytochrome c fibril formation is of primary importance for understanding its misfunctioning and, in a wider perspective, for its technologic applications in the field of bio-nanoscience. In this work, we analyzed the morphology and the spectroscopic properties of cytochrome c aggregates, combining the outcomes from electron microscopy, fluorescence, infrared and Raman spectroscopies and making use of statistical tools for the data analysis. The morphology scenario is quite complex, as it points out the presence of aggregates in the shape of platelets as well as fibers at micrometric scale. By infrared and Raman spectroscopy we analyzed the secondary and tertiary structures of unordered aggregates and fibrils, drawing a pathway for their formation at the timescales from tenths to hundreds of minutes. Dependence of the fibrillation route on environmental pH, above and below the isoelectric point, and on protein concentration has also been explored. We found ...
Source: International Journal of Biological Macromolecules - Category: Biochemistry Authors: Tags: Int J Biol Macromol Source Type: research