4'-Guanidinium-modified siRNA: a molecular tool to control RNAi activity through RISC priming and selective antisense strand loading.

4'-Guanidinium-modified siRNA: a molecular tool to control RNAi activity through RISC priming and selective antisense strand loading.
Chem Commun (Camb). 2019 Jul 12;:
Authors: Nawale GN, Bahadorikhalili S, Sengupta P, Kadekar S, Chatterjee S, Varghese OP
Abstract
We designed novel 4'-C-guanidinocarbohydrazidomethyl-5-methyl uridine (GMU) modified small interfering RNA (siRNA) and evaluated its biophysical and biochemical properties. Incorporation of GMU units significantly increased the thermodynamic stability as well as the enzymatic stability against nucleases in human serum. A gene silencing experiment indicated that GMU modfied siRNA (siRNA6) resulted in ≈4.9-fold more efficient knockdown than unmodified siRNA.
PMID: 31298670 [PubMed - as supplied by publisher]

Source: Chemical Communications - July 11, 2019 Category: Chemistry Authors: Nawale GN, Bahadorikhalili S, Sengupta P, Kadekar S, Chatterjee S, Varghese OP Tags: Chem Commun (Camb) Source Type: research