Microbial dysbiosis in inflammatory bowel diseases: results of a metagenomic study in Saudi Arabia.

CONCLUSIONS: A significant dysbiosis was found in UC and CD patients compared to controls. PMID: 31293117 [PubMed - as supplied by publisher]
Source: Minerva Gastroenterologica e Dietologica - Category: Gastroenterology Tags: Minerva Gastroenterol Dietol Source Type: research

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Publication date: Available online 25 August 2019Source: Journal of Microbiological MethodsAuthor(s): Guerrino Macori, Siobhán C. McCarthy, Catherine M. Burgess, Séamus Fanning, Geraldine DuffyAbstractA quantitative PCR method is described for the detection and quantification of E. coli O157 and O26 in sheep recto-anal junction swabs. The method incorporated a short enrichment step (5 h) and the use of a developed standard calibration curve relating the real time PCR cycle threshold (Ct) values against the initial concentration of pathogen in the sheep sample.
Source: Journal of Microbiological Methods - Category: Microbiology Source Type: research
Abstract The aim of the study was to find out the carbapenem resistance rate and prevalence of different carbapenemase genes in Klebsiella pneumoniae and Escherichia coli from a North Indian corporate hospital that receives both Indian and international patients. A total of 528 clinical isolates of E. coli and K. pneumoniae were included in the study. All isolates that were found resistant to carbapenems by MIC testing (Vitek II Compact®) were screened for NDM, OXA-48, VIM, and KPC genes by PCR. Sequencing of NDM gene and transmissibility by conjugation assay were checked on 22 randomly selected NDM-positive i...
Source: Acta Microbiologica et Immunologica Hungarica - Category: Microbiology Authors: Tags: Acta Microbiol Immunol Hung Source Type: research
In this study, an ApoA-I gene named BbApoA-I having an open reading frame of 1466 bp and encoding a polypeptide of 345 amino acids was cloned from a cephalochordate (Branchiostoma belcheri). The predicted polypeptide revealed conserved features, including α-helical secondary structures and coiled-coil regions in known vertebrate ApoA-I sequences. Quantitative real-time PCR analysis indicated that BbApoA-I was detected in all of the tested tissues, and the highest expression was found in the hepatic cecum. The BbApoA-I expression was upregulated after the specimens were injected with lipopolysaccharide. Recombinant ...
Source: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology - Category: Molecular Biology Source Type: research
We are now heading into an extensive bout of lawgiving. I won't start in this chapter, which is just the warmup. Let me take a brief aside to refer you to theAwkward  Moments Children's Bible. We obviously won't get to Samuel for a while so this will give you a taste of what's ahead once the Israelites have kings and all that.As I often note here, the parts of the Bible they teach in Sunday school, and the parts that get preached about, are highly selective. In fact Tufts professor Daniel Dennetthas written a whole book about ministers who lost their faith when they went to the theological seminary and actually read t...
Source: Stayin' Alive - Category: American Health Source Type: blogs
Publication date: 5 January 2020Source: Journal of Molecular Structure, Volume 1199Author(s): Anindita De, Hari Prakash Ray, Preeti Jain, Harsimrut Kaur, Nikita SinghAbstractSchiff base ligand (E)-2-(2-hydroxy-3-methoxybenzylideneamino)acetic acid) and its Mn(II), Fe(II),Co(III),Ni (II), Cu(II) and Zn(II) complexes were synthesized and characterized by elemental analysis, UV–Vis, IR, 1H NMR, EPR and X-ray crystallography techniques. The ligand and its metal complexes were evaluated for their DNA cleavage activity using E. Coli genome by agarose gel electrophoresis method. Molecular docking studies performed for ...
Source: Journal of Molecular Structure - Category: Molecular Biology Source Type: research
Publication date: Available online 23 August 2019Source: Carbohydrate PolymersAuthor(s): Yueying Wang, Minhao Xie, Gaoxing Ma, Yong Fang, Wenjian Yang, Ning Ma, Donglu Fang, Qiuhui Hu, Fei PeiAbstractFour phenolic acid-grafted-chitosan conjugates were prepared with different phenolic acids including gallic acid (GLA), caffeic acid (CA), gentisate acid (GTA) and sinapic acid (SA), and their characteristics were analyzed. Furthermore, the antioxidant and antimicrobial activities of conjugates were compared to screen out the optimal conjugates. The characterization analysis showed that the UV absorption peaks of CA-g-CS were ...
Source: Carbohydrate Polymers - Category: Biomedical Science Source Type: research
Publication date: Available online 23 August 2019Source: Journal of Organometallic ChemistryAuthor(s): Umie F.M. Haziz, Rosenani A. Haque, A.A. Amirul, O.Noor Aidda, Mohd R. RazaliAbstractThis work describes the synthesis of an uncommon non-symmetrical dibenzimidazolium salts as a precursor for the dinuclear Ag(I)–NHC complexes with a formula of [Ag2L2]‧2PF6 (L = NHC = bis-N-heterocyclic carbene). Through the reaction of 3-(2-bromoethyl)-1-butylbenzimidazole bromide (i) with n-alkylbenzimidazole (alkyl = methyl, ethyl, propyl, pentyl, hexyl, heptyl, benzyl), seven non-symmetrical dibenzimidazolium bromi...
Source: Journal of Organometallic Chemistry - Category: Chemistry Source Type: research
Publication date: Available online 24 August 2019Source: Journal of Environmental Chemical EngineeringAuthor(s): Camila Suliani Raota, Aline Fagundes Cerbaro, Mirian Salvador, Ana Paula Longaray Delamare, Sergio Echeverrigaray, Janaina da Silva Crespo, Thiago Barcellos da Silva, Marcelo GiovanelaAbstractTraditional methods for synthesizing metallic nanoparticles include the use of toxic chemicals, which can have negative impacts on the environment and human health. A safer alternative to produce these nanomaterials is based on the concept of Green Chemistry, which uses less harmful chemicals from renewable sources. In this...
Source: Journal of Environmental Chemical Engineering - Category: Chemistry Source Type: research
In conclusion, the results obtained in this study demonstrate that photodynamic therapy using blue LED light interferes with the antibacterial action of P. arboreum, P. aduncum and P. gaudichaudianum essential oils and aminoglycoside antibiotic activity.
Source: Journal of Photochemistry and Photobiology B: Biology - Category: Speech-Language Pathology Source Type: research
Conclusions: The study documented the low level of BC knowledge among male partners. There is an urgent need to pay more consideration to disseminating awareness among men, as they are associates, and they must be armed with proper awareness. There is an urgent demand for establishing a national program and educational campaigns towards BC. Stressing the advantages and access to free mammography is necessary.
Source: International Journal of Environmental Research and Public Health - Category: Environmental Health Authors: Tags: Article Source Type: research
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