DNA Import into Plant Mitochondria: Complex Approach for in organello and in vivo Studies

The objective of this work was to improve and standardize the existing approaches for investigating DNA import into plant mitochondria in anin organello system. A method for detecting the import of fluorescently labeled DNA substrates has been developed. Based on the features of DNA import into the mitochondria, we suggested an efficient method for the evaluation of the DNA import efficiency by quantitative PCR. We also developed and characterized thein vivo system that allows to detect DNA transport from the cytoplasm to the mitochondrial matrix inArabidopsis thaliana protoplasts. A combination of the proposed techniques for studying the DNA uptake by plant mitochondria might be useful for elucidating whether the properties of the mitochondrial DNA import established in thein organello system are preservedin vivo.
Source: Biochemistry (Moscow) - Category: Biochemistry Source Type: research