Pain inhibition through transplantation of fetal neuronal progenitors into the injured spinal cord in rats.

Pain inhibition through transplantation of fetal neuronal progenitors into the injured spinal cord in rats. Neural Regen Res. 2019 Nov;14(11):2011-2019 Authors: Batista CM, Mariano ED, Dale CS, Cristante AF, Britto LR, Otoch JP, Teixeira MJ, Morgalla M, Lepski G Abstract Neuropathic pain after spinal cord injury (SCI) is a complex condition that responds poorly to usual treatments. Cell transplantation represents a promising therapy; nevertheless, the ideal cell type in terms of neurogenic potential and effectiveness against pain remains largely controversial. Here, we evaluated the ability of fetal neural stem cells (fNSC) to relieve chronic pain and, secondarily, their effects on motor recovery. Adult Wistar rats with traumatic SCI were treated, 10 days after injury, with intra-spinal injections of culture medium (sham) or fNSCs extracted from telencephalic vesicles (TV group) or the ventral medulla (VM group) of E/14 embryos. Sensory (von Frey filaments and hot plate) and motor (the Basso, Beattie, Bresnahan locomotor rating scale and inclined plane test) assessments were performed during 8 weeks. Thereafter, spinal cords were processed for immunofluorescence and transplanted cells were quantified by stereology. The results showed improvement of thermal hyperalgesia in the TV and VM groups at 4 and 5 weeks after transplantation, respectively. Moreover, mechanical allodynia improved in both the TV and VM groups at 8 weeks. No significant motor recovery...
Source: Cell Research - Category: Cytology Authors: Tags: Neural Regen Res Source Type: research

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Abstract Spinal cord injury (SCI) causes maladaptive changes to nociceptive synaptic circuits within the injured spinal cord. Changes also occur at remote regions including the brain stem, limbic system, cortex, and dorsal root ganglia. These maladaptive nociceptive synaptic circuits frequently cause neuronal hyperexcitability in the entire nervous system and enhance nociceptive transmission, resulting in chronic central neuropathic pain following SCI. The underlying mechanism of chronic neuropathic pain depends on the neuroanatomical structures and electrochemical communication between pre- and postsynaptic neuro...
Source: Cellular and Molecular Neurobiology - Category: Cytology Authors: Tags: Cell Mol Neurobiol Source Type: research
DiscussionThis is the first case, to our knowledge, which reports the successful use of SCS in a patient with spinal dAVF-related pain. Spinal vascular malformations, even when cured with treatment, may result in permanent spinal cord damage and engender chronic neuropathic pain for which SCS below the level of injury may be considered.
Source: Interdisciplinary Neurosurgery - Category: Neurosurgery Source Type: research
Publication date: Available online 9 January 2020Source: Neurochemistry InternationalAuthor(s): Tasuku Nishihara, Junya Tanaka, Keisuke Sekiya, Yuki Nishikawa, Naoki Abe, Taisuke Hamada, Sakiko Kitamura, Keizo Ikemune, Shinichiro Ochi, Mohammed E. Choudhury, Hajime Yano, Toshihiro YorozuyaAbstractChronic constriction injury of the sciatic nerve is frequently considered as a cause of chronic neuropathic pain. Marked activation of microglia in the posterior horn (PH) has been well established with regard to this pain. However, microglial activation in the anterior horn (AH) is also strongly induced in this process. Therefore...
Source: Neurochemistry International - Category: Neuroscience Source Type: research
(The Physiological Society) New research shows how second-degree burns cause hard-to-treat chronic pain, and this understanding may be key to treating these complications, common in war veterans This research, published in Physiological Reports, suggests that burns cause changes to neurons in multiple parts of the spinal cord, even far from the injury site, which can contribute to chronic pain and other long-term complications.
Source: EurekAlert! - Medicine and Health - Category: International Medicine & Public Health Source Type: news
Abstract A growing body of evidence has indicated that the release of nociceptive factors, such as interleukins and chemokines, by activated immune and glial cells has crucial significance for neuropathic pain generation and maintenance. Moreover, changes in the production of nociceptive immune factors are associated with low opioid efficacy in the treatment of neuropathy. Recently, it has been suggested that CC chemokine receptor type 1 (CCR1) signaling is important for nociception. Our study provides evidence that the development of hypersensitivity in rats following chronic constriction injury (CCI) of the scia...
Source: Immunology - Category: Allergy & Immunology Authors: Tags: Immunology Source Type: research
Publication date: Available online 7 January 2020Source: Brain, Behavior, and ImmunityAuthor(s): Shahani Noor, Joshua J. Sanchez, Melody S. Sun, Zinia Pervin, Jacob E. Sanchez, Mara A. Havard, Lauren T. Epler, Monique V. Nysus, Jeffrey P. Norenberg, Carston R. Wagner, Suzy Davies, Wagner Jennifer L, Daniel D. Savage, Lauren L. Jantzie, Nikolaos Mellios, Erin.D. MilliganAbstractPrevious reports show that moderate prenatal alcohol exposure (PAE) poses a risk factor for developing neuropathic pain following adult-onset peripheral nerve injury in male rats. Recently, evidence suggests that immune-related mechanisms underlying ...
Source: Brain, Behavior, and Immunity - Category: Neurology Source Type: research
Abstract Previous reports show that moderate prenatal alcohol exposure (PAE) poses a risk factor for developing neuropathic pain following adult-onset peripheral nerve injury in male rats. Recently, evidence suggests that immune-related mechanisms underlying neuropathic pain in females are different compared to males despite that both sexes develop neuropathy of similar magnitude and duration following chronic constriction injury (CCI) of the sciatic nerve. Data suggest that the actions of peripheral T cells play a greater role in mediating neuropathy in females. The goal of the current study is to identify specif...
Source: Brain, Behavior, and Immunity - Category: Neurology Authors: Tags: Brain Behav Immun Source Type: research
Abstract BACKGROUND: Pulsed radiofrequency (PRF), as a non-invasive treatment of neuropathic pain (NP), has been widely administered clinically. Previous studies have shown that PRF has the potential to improve hyperalgesia in animal models of NP. However, there have been few reports to clarify whether the mechanism of PRF treatment of NP involves intervention in the expression of substance P (SP). Therefore, this study administered PRF treatment to chronic constriction injury (CCI) model rats and observed the sciatic nerve mechanical pain threshold and SP expression in the spinal cord to explore the mechanism of ...
Source: Chinese Medical Journal - Category: General Medicine Authors: Tags: Chin Med J (Engl) Source Type: research
Abstract OBJECTIVE: To explore the analgesic mechanism of intrathecal trichostatin A (TSA) injection in a rat model of neuropathic pain induced by chronic constrictive injury (CCI). METHODS: Male SD rats were randomized into sham operation+ DMSO group (group S), CCI +DMSO group (group C), CCI +10 μg TSA group (group T), and in the latter two groups, rat models of neuropathic pain were established induced by CCI. The rats were given intrathecal injections of 10 μL 5% DMSO or 10 μg TSA (in 5% DMSO) once a day on days 7 to 9 after CCI or sham operation. The rats were euthanized after behavioral tests on...
Source: Journal of Southern Medical University - Category: Universities & Medical Training Authors: Tags: Nan Fang Yi Ke Da Xue Xue Bao Source Type: research
cute;csei L Abstract Neuropathic pain is a chronic secondary pain condition, which is a consequence of peripheral or central nervous (somatosensory) system lesions or diseases. It is a devastating condition, which affects around 7% of the general population. Numerous etiological factors contribute to the development of chronic neuropathic pain. It can originate from the peripheral part of the nervous system such as in the case of trigeminal or postherpetic neuralgia, peripheral nerve injury, painful polyneuropathies, or radiculopathies. Central chronic neuropathic pain can develop as a result of spinal cord or bra...
Source: Behavioural Neurology - Category: Neurology Authors: Tags: Behav Neurol Source Type: research
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