Clonality testing as complementary tool in the assessment of different patient groups with canine chronic enteropathy

Publication date: Available online 10 July 2019Source: Veterinary Immunology and ImmunopathologyAuthor(s): Nicole Luckschander-Zeller, Sabine E. Hammer, Barbara C. Ruetgen, Alexander Tichy, Johann G. Thalhammer, E. Haas, Barbara Richter, Monika Welle, Iwan A. BurgenerAbstractDifferentiation between canine chronic enteropathy (CCE) and intestinal lymphoma is a diagnostic challenge as histopathology might fail to yield unequivocal results. Detection of clonal rearrangements of the T-cell-receptor gamma (TCRG) chain and IG heavy chain (IGH) V-J genes offer a useful solution. In this retrospective study, histopathology samples of 35 CCE patients and 7 healthy Beagle dogs underwent clonality testing. Patients suffered either from inflammatory bowel disease (IBD), food responsive diarrhea (FRD) or protein loosing enteropathy secondary to IBD (PLE/IBD). Healthy Beagles served as controls (CO).Canine IBD activity index (CIBDAI) and histopathological WSAVA–grading differed significantly (p<0.001) between groups. CIBDAI improved significantly after appropriate therapy (p < 0.0001). Intestinal biopsies of all CO showed polyclonal patterns for B- and T-cell primers. All samples from CCE patients showed polyclonal patterns for the B-cell primers. Targeting TCRG, 4 patients showed a monoclonal or oligoclonal pattern of the lymphocytic infiltrates in the duodenum and/or colon. Clinical improvement was observed in all dogs. Although a small cell lymphoma cannot be excluded in v...
Source: Veterinary Immunology and Immunopathology - Category: Veterinary Research Source Type: research