Endocannabinoid and N-acylethanolamide levels in rat brain and spinal cord following systemic dipyrone and paracetamol administration.

Endocannabinoid and N-acylethanolamide levels in rat brain and spinal cord following systemic dipyrone and paracetamol administration. Can J Physiol Pharmacol. 2019 Jul 08;: Authors: Topuz RD, Gunduz O, Karadag HC, Dokmeci D, Ulugol A Abstract Contribution of cannabinoid system has been suspected to play role in the mechanisms of actions of dipyrone and paracetamol. Our purpose was to measure the local endocannabinoid and N-acylethanolamide levels in brain and spinal cord of rats following dipyrone and paracetamol administration. In this project, nociception tests were assessed 1-, 5- and 12-hours following drug injections in Wistar rats, using tail-flick and hot-plate apparatus. Firstly, antinociceptive effects of dipyrone (150, 300, 600 mg/kg, i.p.) and paracetamol (30, 100, 300 mg/kg, i.p.) are observed. Then, after administration of highest doses of dipyrone and paracetamol, endocannabinoid (N-arachidonoylethanolamide [AEA], 2-arachidonoylglycerol [2-AG]) and N-acylethanolamine (palmitoylethanolamide [PEA], oleoylethanolamide [OEA]) levels are measured in the periaqueductal gray (PAG), the rostral ventromedial medulla (RVM) and the spinal cords of rats, using LC-MS/MS. Increased 2-AG levels are observed in the PAG and the RVM 12 hours after dipyrone injection; paracetamol exerted no action on 2-AG levels. Analgesic administrations lead to reductionn AEA levels in the RVM and the spinal cord; similar decreases in PEA and OEA level...
Source: Canadian Journal of Physiology and Pharmacology - Category: Drugs & Pharmacology Authors: Tags: Can J Physiol Pharmacol Source Type: research