Integrated Glycome Strategy for Characterization of Aberrant LacNAc Contained N-Glycans Associated With Gastric Carcinoma

Aberrant glycosylation is not only a feature of malignant cell transformation but also plays an important role in metastasis. In present study, an integrated strategy combining the lectin microarrays and lectin histochemistry was employed to investigate and verify the altered glycopatterns in gastric cancer (GC) cell lines as well as tumor specimens from matched tissue sets of 46 autopsy GC patients with the different stages. Subsequently, lectin-mediated affinity capture glycoproteins and MALDI-TOF/TOF-MS were also employed to acquire precise structural information of the altered glycans. According to the results, the glycopatterns recognized by 10 (e.g., ACA, MAL-I and ConA) and 3 lectins (PNA, MAL-I and VVA) showed significantly altered in GC cells and tissues compared to their corresponding controls, respectively. Notably, the glycopattern of GalĪ²-1,4GlcNAc (LacNAc) recognized by MAL-I exhibited significantly increased in GC cells (p
Source: Frontiers in Oncology - Category: Cancer & Oncology Source Type: research