An approach to recombinantly produce mature grape polyphenol oxidase.

An approach to recombinantly produce mature grape polyphenol oxidase.
Biochimie. 2019 Jul 05;:
Authors: Li Y, McLarin MA, Middleditch MJ, Morrow SJ, Kilmartin PA, Leung IKH
Abstract
Polyphenol oxidases (PPOs) are important enzymes that are widely found in both prokaryotes and eukaryotes including grapes. Studies of grape PPO to date have mostly relied on enzymes extracted and purified from plants. In this work, we describe the production of the mature form of Shine Muscat grape PPO by using an Escherichia coli expression system. We have optimised the purification procedure to obtain pure and active recombinant enzymes and characterised the catalytic efficiency of the recombinant grape PPO by using ultraviolet/visible (UV/Vis) spectrophotometry. Our work provides a simple protocol of obtaining pure and active recombinant grape PPO that will enable further studies about the catalytic mechanism and inhibition of this enzyme.
PMID: 31283975 [PubMed - as supplied by publisher]

Source: Biochimie - July 4, 2019 Category: Biochemistry Authors: Li Y, McLarin MA, Middleditch MJ, Morrow SJ, Kilmartin PA, Leung IKH Tags: Biochimie Source Type: research