Life-time exposure to waterborne copper III: Effects on the energy metabolism of the killifish Poecilia vivipara.

Life-time exposure to waterborne copper III: Effects on the energy metabolism of the killifish Poecilia vivipara. Chemosphere. 2019 Jul;227:580-588 Authors: Abou Anni IS, Zebral YD, Afonso SB, Moreno Abril SI, Lauer MM, Bianchini A Abstract Copper ions (Cu) are essential to life maintenance, nonetheless, elevated concentrations can be hazardous. Acute and sub-chronic toxic effects of this metal are well known and are usually related to enzymatic inhibition, elevated ROS production and dysfunction of energy metabolism. Despite that, chronic studies are extremely rare. Therefore, the aim of this study was to assess the effects of chronic exposure to 5, 9 and 20 μg/L Cu (28 ad 345 days) on the energy metabolism and survival of the killifish Poecilia vivipara. To accomplish that, we evaluated the activity of enzymes related to aerobic (pyruvate kinase (PK); citrate synthase (CS)) and anaerobic metabolism (lactate dehydrogenase (LDH)) in whole-body (28 days) or in gills, liver and muscle (345 days) of exposed fish. Additionally, whole-body oxygen consumption was evaluated in fish exposed for 28 days and hepatic and muscular expression of genes involved in mitochondrial metabolism (cox I, II and III and atp5a1) was assessed in animals exposed for 345 days. Finally, final survival was evaluated. Following 28 days, Cu did not affect survival neither enzyme activities. However, increased whole-body oxygen consumption was observed in compar...
Source: Chemosphere - Category: Chemistry Authors: Tags: Chemosphere Source Type: research