Protective effect of 3-bromo-4,5-dihydroxybenzaldehyde from Polysiphonia morrowii Harvey against hydrogen peroxide-induced oxidative stress in in vitro and in vivo model zebrafish.

Protective effect of 3-bromo-4,5-dihydroxybenzaldehyde from Polysiphonia morrowii Harvey against hydrogen peroxide-induced oxidative stress in in vitro and in vivo model zebrafish. J Microbiol Biotechnol. 2019 Jul 09;: Authors: Cho SH, Heo SJ, Yang HW, Ko EY, Jung MS, Cha SH, Ahn G, Jeon YJ, Kim KN Abstract We investigated the protective effects of 3-bromo-4,5-dihydroxybenzaldehyde (BDB) from Polysiphonia morrowii Harvey against hydrogen peroxide (H2O2)-induced apoptosis in Vero cells. BDB exhibited scavenging activity for DPPH, hydroxyl, and alkyl radicals. BDB also inhibited H2O2-induced lipid peroxidation, cell death, and apoptosis in Vero cells by inhibiting the production of ROS. To evaluate the molecular mechanisms of apoptosis inhibition, the expression of Bax/Bcl-xL and NF-κB was assessed by western blot assay. BDB significantly inhibited the cleavage of caspase-9 and PARP and reduced Bax levels in H2O2-induced Vero cells. In addition, BDB suppressed the phosphorylation of NF-κB and the translocation of p65 in H2O2-induced cells. Furthermore, we evaluated the effect of BDB on ROS production, cell death, and lipid peroxidation in a H2O2-stimulated zebrafish embryo model. The results indicated that ROS generation and cell death were significantly inhibited by BDB in zebrafish embryos. These results prove that BDB has excellent antioxidant activity in vitro and in vivo. PMID: 31280521 [PubMed - as supplied by publisher...
Source: Journal of Microbiology and Biotechnology - Category: Biotechnology Authors: Tags: J Microbiol Biotechnol Source Type: research