Purification and enzymatic characterization of the RNA ligase RTCB from Thermus thermophilus.

CONCLUSION: D64 was proved to be essential for RTCB-catalyzed RNA ligation and DNA activation (from 37 to 70 °C) in T. thermophilus.
PMID: 31280403 [PubMed - as supplied by publisher]

Source: Biotechnology Letters - July 5, 2019 Category: Biotechnology Authors: Duan S, Chen Z, Li Z, Ji R, Gan J, Li J Tags: Biotechnol Lett Source Type: research