Carboxypeptidase A4 promotes cell growth via activating STAT3 and ERK signaling pathways and predicts a poor prognosis in colorectal cancer.

In this study, we investigated the functions and mechanisms by which CPA4 promotes CRC progression. Quantitative real-time PCR (qRT-PCR) and western blot showed that CPA4 mRNA and CPA4 protein levels were up-regulated in CRC compared to levels in adjacent normal tissue. Immunohistochemistry (IHC) results indicating high CPA4 levels were positively associated with poor prognoses. In addition, Cell Counting Kit-8 (CCK-8), colony formation, flow cytometry, and transwell assays demonstrated that CPA4 overexpression facilitated the growth of CRC cells, whereas CPA4 knockdown resulted in decreased proliferation, G1/S phase transition arrest, and apoptosis. Subcutaneous tumorigenesis was performed in nude mice to confirm the tumor-promoting effects of CPA4 in vivo. Western blot revealed that activation of the STAT3 and ERK pathways is one of the oncogenic functions of CPA4 in CRC. Accordingly, CPA4 promotes CRC cell growth via activating the STAT3 and ERK pathways and may be a prognostic factor or therapeutic target for CRC. PMID: 31279884 [PubMed - as supplied by publisher]
Source: International Journal of Biological Macromolecules - Category: Biochemistry Authors: Tags: Int J Biol Macromol Source Type: research

Related Links:

Publication date: Available online 24 August 2019Source: Hepatobiliary &Pancreatic Diseases InternationalAuthor(s): Yan-Dong Li, Zhen Lv, Hai-Yang Xie, Shu-Sen ZhengAbstractBackgroundRetinoblastoma binding protein 4 (RBBP4) plays an essential role in the development of multiple cancers. However, its relationship with prognosis in colon cancer and colon cancer hepatic metastasis has not been elucidated. The aim of this study was to explore the relationship between RBBP4 expression and prognosis of colon cancer patients and to evaluate RBBP4 as a new prognostic marker in these patients.MethodsEighty colon cancer patients...
Source: Hepatobiliary and Pancreatic Diseases International - Category: Gastroenterology Source Type: research
This study, for the first time, aims to quantify the impact of modulation restriction on plan quality indices in inverse optimization for cervix high-dose-rate (HDR) brachytherapy using the BEBIG SagiPlan treatment planning system. Material and methods: Ten cervical cancer patient plans were optimized for treatment with a BEBIG SagiNova 60Co HDR afterloader using the min/max inverse planning method, with dwell time homogeneity error weight (DTHEW) parameter values of 0 to 10. Dwell time homogeneity and gradients as well as various plan quality indices were analyzed. Results: For DTHEW = 0, min/max-based optimizatio...
Source: Journal of Contemporary Brachytherapy - Category: Cancer & Oncology Tags: J Contemp Brachytherapy Source Type: research
Dehydrogenase/reductase SDR family member 2 silencing sensitizes an oxaliplatin‑resistant cell line to oxaliplatin by inhibiting excision repair cross‑complementing group 1 protein expression. Oncol Rep. 2019 Aug 22;: Authors: Li JM, Jiang GM, Zhao L, Yang F, Yuan WQ, Wang H, Luo YQ Abstract Oxaliplatin (Oxa)‑based chemotherapy is widely used as the first‑line treatment for colorectal cancer (CRC). However, Oxa‑resistance is common for many postoperative CRC patients. To explore drug resistance in CRC, an Oxa‑resistant cell line, HCT116/Oxa, was established from parental HCT116 cells. ...
Source: Oncology Reports - Category: Cancer & Oncology Tags: Oncol Rep Source Type: research
ConclusionsHepatectomy is a safe and effective way of treating breast cancer liver metastasis at experienced centers where multidisciplinary adjuvant treatments are available. It can be considered more frequently as part of the multidisciplinary care for this patient population.
Source: Hepatobiliary and Pancreatic Diseases International - Category: Gastroenterology Source Type: research
ConclusionIn conclusion, ACT does not provide survival benefits for patients after resection of PM from CRC. ACT and targeted agents (BV) are not suggested for these patients.
Source: International Journal of Colorectal Disease - Category: Gastroenterology Source Type: research
Invasive mucinous adenocarcinoma (IMA) is a variant of lung adenocarcinoma [1]. The tumor cells in IMA are columnar and contain abundant intracytoplasmic mucin, mimicking goblet cells of the colon [1]. IMA is often positive for HNF4-alpha, but negative for TTF-1 [1]. IMA reveals genetic abnormalities similar to those of colorectal carcinoma and commonly shows KRAS mutation (in up to 90% of cases) [1,2].
Source: Lung Cancer - Category: Cancer & Oncology Authors: Source Type: research
Quality control can decrease variations among colonoscopists ’ performance and improve colonoscopy effectiveness to prevent colorectal cancers. Unfortunately, routine quality control is difficult to carry out because of lacking a practical method. The aim of this study was to develop an automatic quality control system (AQCS) and assess whether it could imp rove polyp and adenoma detection in clinical practice.
Source: Gastrointestinal Endoscopy - Category: Gastroenterology Authors: Source Type: research
Publication date: Available online 22 August 2019Source: Life SciencesAuthor(s): Yang Ge, Bao-lin Liu, Jun-peng Cui, Shu-qiang LiAbstractLivin is an important member of the human inhibitor of apoptosis proteins (IAPs) family. IAPs are proteins with antiapoptotic abilities, and their functions are different from the Bcl-2 (B-cell lymphoma-2) family proteins. However, the precise role of Livin in colon cancer progression remains unclear. The purpose of this study is to assess the effect of overexpression Livin in colon cancer cells and to examine its molecular mechanism. We demonstrated that Livin induced a colon cancer phen...
Source: Life Sciences - Category: Biology Source Type: research
The objective of this study was to evaluate the anticancer activity on cancer cell models of a drug delivery system consisting of poly (l-lactic) acid/Pluronic® F-127 (PLLA/PF127) loaded with the new N-butylpyridoquinoxaline 1,4-dioxide (NBPQD) or 2-amino-3-cyano-6-methylquinoxaline 1,4-dioxide (ACMQD) that was synthesized using an electrospinning process compared to free NBPQD and ACMQD. PLLA/PF127-NBPQD and PLLA/PF127-ACMQD nanofibers were prepared, and their shape, size, Fourier-transform infrared spectroscopy (FTIR), thermogravimetric (TGA) analysis, water contact angel (WCA), drug release, anticancer activity agai...
Source: Colloids and Surfaces B: Biointerfaces - Category: Biochemistry Source Type: research
Mutational inactivation of p53 is a key player in the development of human cancer. Thus, retrieving the tumor suppressor activity of p53 gene is considered a novel strategy in cancer therapy. Current study aimed to investigate the anti-cancer potentials of botulinum toxin type-A (BTX-A) and captopril as a trial to shed light on effective anti-cancer therapy with lower side effects. Cytotoxic effect of captopril and BTX-A was determined using MTT assay against colon (HCT116) and prostate cancer (DU145) cells compared to their effect on normal vero cells. Anti-proliferation assay and anti-metastatic effect were carried out u...
Source: Iranian Journal of Pharmaceutical Research - Category: Drugs & Pharmacology Source Type: research
More News: Biochemistry | Cancer | Cancer & Oncology | Colorectal Cancer | Genetics | Study