A model for the chemomechanical coupling of the mammalian cytoplasmic dynein molecular motor.

A model for the chemomechanical coupling of the mammalian cytoplasmic dynein molecular motor.
Eur Biophys J. 2019 Jul 05;:
Authors: Xie P
Abstract
Available single-molecule data have shown that some mammalian cytoplasmic dynein dimers move on microtubules with a constant step size of about 8.2 nm. Here, a model is presented for the chemomechanical coupling of these mammalian cytoplasmic dynein dimers. In contrast to the previous models, a peculiar feature of the current model is that the rate constants of ATPase activity are independent of the external force. Based on this model, analytical studies of the motor dynamics are presented. With only four adjustable parameters, the theoretical results reproduce quantitatively diverse available single-molecule data on the force dependence of stepping ratio, velocity, mean dwell time, and dwell-time distribution between two mechanical steps. Predicted results are also provided for the force dependence of the number of ATP molecules consumed per mechanical step, indicating that under no or low force the motors exhibit a tight chemomechanical coupling, and as the force increases the number of ATPs consumed per step increases greatly.
PMID: 31278451 [PubMed - as supplied by publisher]

Source: European Biophysics Journal : EBJ - July 4, 2019 Category: Physics Authors: Xie P Tags: Eur Biophys J Source Type: research