Label-free localized surface plasmon resonance biosensor composed of multi-functional DNA 3 way junction on hollow Au spike-like nanoparticles (HAuSN) for avian influenza virus detection

Publication date: 1 October 2019Source: Colloids and Surfaces B: Biointerfaces, Volume 182Author(s): Taek Lee, Ga Hyeon Kim, Soo Min Kim, Keonyoung Hong, Younghun Kim, Chulhwan Park, Hiesang Sohn, Junhong MinAbstractIn the present study, we fabricated a label-free avian influenza (AIV H5N1) detection biosensor composed of a multi-functional DNA 3 way-Junction (3 W J) on a hollow Au spike-like nanoparticle (hAuSN) using a localized surface plasmon resonance (LSPR) method. To construct the multi-functional DNA (MF-DNA) as a bioprobe, the 3 W J was introduced. The proposed AIV detection bioprobe should contain three functionalities: target recognition, signal amplification, and connection to substrate. To achieve this goal, each piece of the DNA 3 W J was tailored to a hemagglutinin (HA) binding aptamer, FAM dye and thiol group, respectively. The assembly of each DNA 3 W J functional fragment was then confirmed by TBM-Native PAGE. Moreover, the hAuSN was immobilized on the indium-tin-oxide (ITO) substrate for LSPR measurement. The DNA 3 W J was immobilized onto the hAuSN electrode through the thiol-group of DNA 3 W J. The fabricated DNA 3 W J/hAuSN heterolayer on the ITO substrate was investigated by field emission scanning electron microscopy (FE-SEM) and atomic force microscopy (AFM). LSPR experiments were conducted to confirm HA protein binding to the DNA 3 W J/ hAu...
Source: Colloids and Surfaces B: Biointerfaces - Category: Biochemistry Source Type: research

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long Xu Janus kinase (JAK) inhibitors have been developed as novel immunomodulatory drugs and primarily used for treating rheumatoid arthritis and other inflammatory diseases. Recent studies have suggested that this category of anti-inflammatory drugs could be potentially useful for the control of inflammation “storms” in respiratory virus infections. In addition to their role in regulating immune cell functions, JAK1 and JAK2 have been recently identified as crucial cellular factors involved in influenza A virus (IAV) replication and could be potentially targeted for antiviral therapy. Gingerenone ...
Source: Viruses - Category: Virology Authors: Tags: Article Source Type: research
Abstract In January 2020, the subclade of highly pathogenic avian influenza (H5N6) virus infected migratory whooper swans and mute swans in Xinjiang, western China. The virus is lethal to chickens and ducks but has low pathogenicity in mice. Antigenically, this subclade is similar to the H5N1 vaccine seed virus Re-11. PMID: 33030424 [PubMed - as supplied by publisher]
Source: Emerging Infectious Diseases - Category: Infectious Diseases Authors: Tags: Emerg Infect Dis Source Type: research
This study was designed to evaluate the persistence of A/H5 HP AIV in slurry from various duck productions. This was achieved i) in the laboratory setting by artificially spiking four AIV-free slurry samples with known amounts of A/H5N9 HP AIV and monitoring virus infectivity, with or without lime treatment to achieve pH 10 or pH 12, and ii) by sampling slurry tanks on five naturally A/H5N8 HP-contaminated farms. Experimental results in artificially spiked slurry suggested virus survival for 4 weeks in slurry from Muscovy- or Pekin-duck breeders, and for 2 weeks in slurry from ducks for "foie-gras" production dur...
Source: Applied and Environmental Microbiology - Category: Microbiology Authors: Tags: Appl Environ Microbiol Source Type: research
Abstract We investigated the experimental infection of two highly pathogenic avian influenza H5N1 viruses isolated from crow (A/crow/Assam/142119/2008) and chicken (A/chicken/Sikkim/151466/2009) in house crows (Corvus splendens). Both viruses caused infection in crows, where four out of six and three out of six crows succumbed to H5N1 infection within 11 days post challenge by crow and chicken viruses, respectively. The major clinical signs in crows were wing paralysis, circling and torticollis. The virus shedding detected from swabs was not persistent in neither crow nor chicken viruses. Both viruses were isolate...
Source: Acta Virologica - Category: Infectious Diseases Authors: Tags: Acta Virol Source Type: research
Publication date: Available online 18 September 2020Source: Veterinary MicrobiologyAuthor(s): Jiao Hu, Ming Kong, Zhu Cui, Zhao Gao, Chunxi Ma, Zenglei Hu, Xinan Jiao, Xiufan Liu
Source: Veterinary Microbiology - Category: Veterinary Research Source Type: research
Ali Stephan Pleschka Egypt is a hotspot for H5- and H9-subtype avian influenza A virus (AIV) infections and co-infections in poultry by both subtypes have been frequently reported. However, natural genetic reassortment of these subtypes has not been reported yet. Here, we evaluated the genetic compatibility and replication efficiency of reassortants between recent isolates of an Egyptian H5N1 and a H9N2 AIV (H5N1EGY and H9N2EGY). All internal viral proteins-encoding segments of the contemporaneous G1-like H9N2EGY, expressed individually and in combination in the genetic background of H5N1EGY, were genetically compati...
Source: Viruses - Category: Virology Authors: Tags: Article Source Type: research
A recent article by Gu in the Journal of Infection(1) reported two highly pathogenic H7N9 strains harboring the E627K mutation in the polybasic protein 2 (PB2) gene from chickens and showed that avian influenza viruses (AIVs) are evolving to adapt to humans. AIVs pose an important and ongoing threat to public health. Historically, both the highly pathogenic avian influenza viruses (H5N1, H5N6, H7N7, H7N3, and H7N9) and the lowly pathogenic avian influenza viruses (H7N2, H7N3, H9N2, H7N9, H6N1, H10N7, and H10N8) have caused sporadic human infections.
Source: Journal of Infection - Category: Infectious Diseases Authors: Tags: Letter to the Editor Source Type: research
AbstractIn 2016/2017, H5N8 highly pathogenic avian influenza (HPAI) virus of the Goose/Guangdong lineage spread from Asia to Europe, causing the biggest and most widespread HPAI epidemic on record in wild and domestic birds in Europe. We hypothesized that the wide dissemination of the 2016 H5N8 virus resulted at least partly from a change in tissue tropism from the respiratory tract, as in older HPAIV viruses, to the intestinal tract, as in low pathogenic avian influenza (LPAI) viruses, allowing more efficient faecal-oral transmission. Therefore, we determined the tissue tropism and associated lesions in wild birds found d...
Source: Veterinary Research - Category: Veterinary Research Source Type: research
gar Simulundu In the recent past, sub-Saharan Africa has not escaped the devastating effects of avian influenza virus (AIV) in poultry and wild birds. This systematic review describes the prevalence, spatiotemporal distribution, and virus subtypes detected in domestic and wild birds for the past two decades (2000–2019). We collected data from three electronic databases, PubMed, SpringerLink electronic journals and African Journals Online, using the Preferred Reporting Items for Systematic reviews and Meta-Analyses protocol. A total of 1656 articles were reviewed, from which 68 were selected. An overall preval...
Source: Viruses - Category: Virology Authors: Tags: Review Source Type: research
We describe the optimal binding features of Oseltamivir derivatives with the SARS-Cov-2 main protease (Code PDB: 6LU7) for further consideration. PMID: 32831522 [PubMed - as supplied by publisher]
Source: Bioinformation - Category: Bioinformatics Authors: Tags: Bioinformation Source Type: research
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