High-Throughput Analytical Light Scattering for Protein Quality Control and Characterization.

We present a review of high-throughput techniques for the characterization and quality control of proteins in the course of purification, evaluation, and formulation, based on static and dynamic light scattering. Multi-angle static light scattering (MALS) in combination with rapid, low-volume UHPLC size exclusion chromatography is effective in characterizing key biophysical properties, while dynamic light scattering (DLS) in high-throughput microwell-plate format provides large quantities of data in a short time to screen many conditions, excipients, cell lines, or candidate biotherapeutics.
PMID: 31267461 [PubMed - in process]

Source: Mol Biol Cell - July 5, 2019 Category: Molecular Biology Authors: Some D, Razinkov V Tags: Methods Mol Biol Source Type: research