Structural insights into the substrate specificity of SP_0149, the substrate-binding protein of a methionine ABC transporter from Streptococcus pneumoniae

Successful pathogenesis is a cumulative effect of the virulence factors of a pathogen and its capability to efficiently utilize the available nutrients from the host. Streptococcus pneumoniae, a Gram-positive opportunistic pathogen, may either reside asymptomatically as a nasopharyngeal commensal inside the human host or cause lethal diseases, including pneumonia, meningitis and sepsis. S. pneumoniae is known to acquire methionine (Met) from its host through a Met importer. Here, the crystal structure of the substrate-binding protein (SBP; SP_0149) of an ABC importer with Met bound is reported at a resolution of 1.95   Å . The three-dimensional structure of SBP shows that it is composed of two distinct domains, each consisting of a mixed β -sheet flanked by helices. The substrate, Met, is bound in the central part of the interface between the two domains. The overall structure of SP_0149 resembles those of SBPs from other reported bacterial Met and Gly-Met dipeptide transporters. However, a detailed analysis of these structures shows notable variations in the amino-acid composition of the substrate-binding pockets of the SP_0149 – Met and GmpC – Gly-Met structures. In particular, SP_0149 harbors Thr212 and Tyr114, whereas the corresponding residues in GmpC are Gly and Val. This difference is likely to be the underlying basis for their differential substrate specificity. In summary, the structure of the SP_0149 – Met complex provides insights int...
Source: Acta Crystallographica Section F - Category: Biochemistry Authors: Tags: Streptococcus pneumoniae ABC transporter substrate-binding protein methionine X-ray crystallography research communications Source Type: research

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Conditions:   Pneumonia;   Meningitis Interventions:   Biological: PCV10;   Biological: PCV13 Sponsor:   University of Witwatersrand, South Africa Recruiting
Source: ClinicalTrials.gov - Category: Research Source Type: clinical trials
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Source: American Family Physician - Category: Primary Care Authors: Tags: Am Fam Physician Source Type: research
In this study, when the distribution of virulence genes were compAred with the isolates that have weak, medium and strong biofilm, all of the studied genes were found to be more abundant in isolates with strong and medium positive biofilm production. This has shown that excluding fimH gene, csgA, csuE and ompA genes have contributed to the biofilm formation in invasive A.baumannii isolates, respectively. PMID: 32050877 [PubMed - in process]
Source: Mikrobiyoloji Bulteni - Category: Microbiology Tags: Mikrobiyol Bul Source Type: research
In this study, a novel DNA-based bioassay was developed to the monitoring of Haemophilus influenza genome in human plasma samples using binding of pDNA with cDNA. DNA hybridization strategy was used to investigation of DNAs binding. For this purpose, silver nanoparticle doped graphene quantum dots inks functionalized by D-penicillamine (Ag NPs-DPA-GQDs) were synthesized and deposited on the surface of glass carbon electrode (GCE). Also, gold nanoparticles functionalized with cysteamine (CysA-AuNPs) were deposited on the surface of the Ag-DPA-GQDs modified GCE. Afterward, thiolated DNA probe was immobilized on the surface o...
Source: International Journal of Biological Macromolecules - Category: Biochemistry Authors: Tags: Int J Biol Macromol Source Type: research
CONCLUSIONS: We detected a small risk of incidental pneumonia associated with varicella vaccine in the 6th week after immunization. There was no increase in the risk of other pre-specified adverse events. PMID: 32046891 [PubMed - as supplied by publisher]
Source: Vaccine - Category: Allergy & Immunology Authors: Tags: Vaccine Source Type: research
(Wolters Kluwer Health) Pneumococcal conjugate vaccines (PCVs) have been highly effective in reducing pneumonia and other invasive infections caused by Streptococcus pneumoniae bacteria. But rates of meningitis have shown little change, as pneumococcal strains not targeted by PCVs emerge as more important causes of meningitis, reports a paper in The Pediatric Infectious Disease Journal, the official journal of The European Society for Paediatric Infectious Diseases. The journal is published in the Lippincott portfolio by Wolters Kluwer.
Source: EurekAlert! - Medicine and Health - Category: International Medicine & Public Health Source Type: news
l;a E Abstract BACKGROUND: Studies on the impact of pneumococcal conjugate vaccines (PCV) on mortality, especially among preschoolers are scarce. We aimed to assess time trend mortality from lower respiratory infections (LRI), taken as a proxy of pneumonia, from 1990 to 2017. METHODS: This ecological study assessed the mortality rate among Brazilians under-five before and after the national PCV-10 implementation in 2010. Pneumococcal meningitis (PM) and diarrheal diseases (DD) were included as a "positive and negative controls", respectively. Trend analysis was performed through Global Burden of Dis...
Source: Vaccine - Category: Allergy & Immunology Authors: Tags: Vaccine Source Type: research
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Source: Eastern Mediterranean Health Journal - Category: Middle East Health Tags: East Mediterr Health J Source Type: research
Publication date: February 2020Source: The Lancet Global Health, Volume 8, Issue 2Author(s): Jeremy D Keenan, Ahmed M Arzika, Ramatou Maliki, Sanoussi Elh Adamou, Fatima Ibrahim, Mariama Kiemago, Nana Fatima Galo, Elodie Lebas, Catherine Cook, Benjamin Vanderschelden, Robin L Bailey, Sheila K West, Travis C Porco, Thomas M Lietman, Paul M Emerson, Jerusha Weaver, Sheila K West, Robin L Bailey, John Hart, Amza AbdouSummaryBackgroundThe Macrolides Oraux pour Réduire les Décès avec un Oeil sur la Résistance (MORDOR) trial found that biannual mass distribution of azithromycin to children younger tha...
Source: The Lancet Global Health - Category: International Medicine & Public Health Source Type: research
Early-onset group B streptococcal (EOGBS) disease (including sepsis, meningitis, and pneumonia) causes significant morbidity and mortality in newborn infants worldwide. Antibiotic prophylaxis can prevent vertical streptococcal transmission, yet no uniform criteria to identify eligible women for prophylaxis exist. Some guidelines recommend universal GBS screening to pregnant women in their third trimester (screening-based protocol), while others employ risk-based protocols. We compare the effectiveness of screening-based vs risk-based protocols in preventing EOGBS disease. Screening-based protocols were associated with lowe...
Source: Current Awareness Service for Health (CASH) - Category: Consumer Health News Source Type: news
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