Hsp genes are differentially expressed during Trichoderma asperellum self-recognition, mycoparasitism and thermal stress

Publication date: Available online 2 July 2019Source: Microbiological ResearchAuthor(s): Thuana Marcolino Mota, Letícia Harumi Oshiquiri, Érica Camelo Viana Lopes, Jomal Rodrigues Barbosa Filho, Cirano José Ulhoa, Raphaela Castro GeorgAbstractHeat shock proteins (Hsp) are important factors in the response of organisms to oscillations in environmental conditions. Although Hsp have been studied for a long time, little is known about this protein class in Trichoderma species. Here we studied the expression of Hsp genes during T. asperellum growth, and mycoparasitism against two phytopathogens: Sclerotinia sclerotiorum and Fusarium oxysporum, as well as during thermal stress. The expression levels of these genes were observed by real-time PCR and they showed to be differentially expressed under these conditions. We verified that the TaHsp26c, TaHsp70b and TaHsp70c genes were differentially expressed over time, indicating that these genes can be developmentally regulated in T. asperellum. Except for TaHsp26a, all other genes analyzed were induced in the post-contact condition when T. asperellum was cultured in a confrontation plate assay against itself. Additionally, TaHsp26b, TaHsp26c, TaHsp90, TaHsp104a and TaHsp104b were induced during initial contact between T. asperellum hyphae, suggesting that these proteins must play a role in the organism´s self-recognition mechanism. When we examined gene expression during mycoparasitism, we observed that some genes were induced both ...
Source: Microbiological Research - Category: Infectious Diseases Source Type: research