Molecular detection of Anaplasma phagocytophilum from larvae of Haemophysalis longicornis in Ibaraki, Japan.

Molecular detection of Anaplasma phagocytophilum from larvae of Haemophysalis longicornis in Ibaraki, Japan. Jpn J Infect Dis. 2019 Jun 28;: Authors: Fukui Y, Inokuma H Abstract DNA from 1084 ticks collected by flagging in vegetation in Tsukuba and Moriya (Ibaraki, Japan), where several cases of canine granulocytic anaplasmosis were found, was molecularly examined for infection with the family Anaplasmataceae. Twenty-six positive samples of Anaplasmataceae-specific PCR of partial 16S rRNA gene were subjected to semi-nested PCR covering the divergent regions the gene and sequence analysis. Anaplasma phagocytophilum was detected in three pools of Haemophysalis longicornis larvae, and Anaplasma bovis from a Haemophysalis flava male. Sequences of both amplicons had highest homologies to those from dogs in our previous studies in Ibaraki, respectively. These results suggest that genus Haemophysalis ticks are the candidate vectors of Anaplasma phagocytophilum and Anaplasma bovis in Ibaraki, Japan. PMID: 31257244 [PubMed - as supplied by publisher]
Source: Japanese Journal of Infectious Diseases - Category: Infectious Diseases Authors: Tags: Jpn J Infect Dis Source Type: research

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In this study, ticks were collected, identified, and pooled (n = 299) from three distinct environments across central Mongolia. Each pool was initially tested for Anaplasma/Ehrlichia using a 16S rRNA PCR assay that detects both genera, and specific PCR testing was done to identify those positive samples. Maximum likelihood estimation (MLE) of infection rates of ticks collected from the environment in Selenge aimag (province) found infection rates of Ixodes persulcatus ticks to be 2.0% (95% CI: 0.7, 4.3%) for A. phagocytophilum and 0.8% (95% CI: 0.1, 2.5%) for both nonspecific Ehrlichia and Anaplasma. Ehrlichi...
Source: Ticks and Tick borne Diseases - Category: Zoology Source Type: research
Abstract Human granulocytic anaplasmosis (HGA), caused by the bacteria Anaplasma phagocytophilum, is transmitted to humans by blacklegged ticks (Ixodes scapularis) in eastern North America. To assess the emergence of A. phagocytophilum in Ontario, we analyzed patient serological and clinical data in combination with pathogen detection in blacklegged ticks from 2011 to 2017. Our sample population included all patients who had Anaplasma serological testing ordered by their physicians (n = 851). Eighty-three patients (10.8%) were A. phagocytophilum seropositive (IgG titers ≥ 1:64) and 686 (89.2%) were seronegative (IgG titers
Source: The American Journal of Tropical Medicine and Hygiene - Category: Tropical Medicine Authors: Tags: Am J Trop Med Hyg Source Type: research
CONCLUSION: We found retrospective molecular evidence of the co-infection of scrub typhus and HGA in Korea. HGA may be more prevalent than expected and need to be considered as an important differential diagnosis of febrile patients in Korea. PMID: 31602827 [PubMed - in process]
Source: Journal of Korean Medical Science - Category: Biomedical Science Tags: J Korean Med Sci Source Type: research
Anaplasma phagocytophilum is an obligate parasitic intracellular bacterium. It is the causative agent of granulocytic anaplasmosis, with effects on human and animal health. In Europe, the pathogen is mainly trans...
Source: Parasites and Vectors - Category: Microbiology Authors: Tags: Short report Source Type: research
Conclusion: Molecular prevalence of A. phagocytophilum was noticeably high. It may cause the incidence of disease in human population. PMID: 31543918 [PubMed]
Source: Iranian Journal of Parasitology - Category: Parasitology Tags: Iran J Parasitol Source Type: research
ska A Abstract PURPOSE: Tick-borne co-infections are a serious epidemiological and clinical problem. Only a few studies aimed to investigate the effect of tick-borne encephalitis (TBE) and human granulocytic anaplasmosis (HGA) co-infection in the course of the inflammatory process and the participation of chemokines in the pathomechanism of these diseases. The aim of the study was to evaluate CCL-4, CCL-17, CCL-20, and IL-8 serum concentrations in patients with HGA, TBE and HGA + TBE co-infection. METHODS: Eighty-seven patients with HGA (n = 20), TBE (n = 49) and HGA + TBE (n = 18) were in...
Source: Cytokine - Category: Molecular Biology Authors: Tags: Cytokine Source Type: research
Publication date: Available online 23 September 2019Source: Ticks and Tick-borne DiseasesAuthor(s): Goudarz Molaei, Eliza AH Little, Kirby C. Stafford, Holly GaffAbstractCases of morphological anomalies in the blacklegged tick, Ixodes scapularis (Acari: Ixodidae), have recently been reported from the Northeastern and upper Midwestern United States, potentially complicating identification of this important vector of human disease-causing pathogens. We hereby report a case of a morphological anomaly in I. scapularis, biting a human host residing in Norwich, Connecticut. Using a dichotomous morphological key, high-resolution ...
Source: Ticks and Tick borne Diseases - Category: Zoology Source Type: research
ConclusionsOur findings indicated thatA.phagocytophilum infection was prevalent but unrecognized in Taiwan.
Source: PLoS Neglected Tropical Diseases - Category: Tropical Medicine Authors: Source Type: research
Publication date: Available online 26 August 2019Source: Ticks and Tick-borne DiseasesAuthor(s): F. Boucher, Y. Moutroifi, B. Peba, M. Ali, Y. Moindjie, A.-S. Ruget, S. Abdouroihamane, Kassim A. Madi, M. Soulé, O. Charafouddine, C. Cêtre-Sossah, E. CardinaleAbstractTick-borne diseases (TBD) occur in many temperate countries and are economically important in most tropical and subtropical areas, affecting dairy and beef cattle, as well as small ruminants. Four major tick-borne diseases have been detected in eastern and southern Africa: East Coast fever (ECF) caused by Theileria parva, Theiler 1904, anaplasmosis ...
Source: Ticks and Tick borne Diseases - Category: Zoology Source Type: research
Publication date: Available online 13 July 2019Source: Small Ruminant ResearchAuthor(s): C. Jiménez, A. Benito, J.L. Arnal, A. Ortín, M. Gómez, A. López, S. Villanueva-Saz, D. LacastaAbstractOvine anaplasmosis is caused by the obligate intraerythrocytic bacteria Anaplasma ovis and the disease is characterized by anaemia, weight loss and weakness. A severe outbreak of ovine anaplasmosis was diagnosed for the first time in Spain in 2014 and, subsequently, a research was developed in order to increase de knowledge of this not-well-known disease.An experimental infection with Anaplasma ovis was carr...
Source: Small Ruminant Research - Category: Zoology Source Type: research
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