Enzymatic Release of Glycoprotein N-Glycans and Fluorescent Labeling.

We present here two robust protocols to achieve N-glycan release. The first one uses trypsin to disrupt protein structure whereas the other involves the use of detergents prior to PNGase F digestion. Thereafter, N-glycans are isolated from peptides using reverse-phase cartridges and are desalted with carbograph cartridges before finally being derivatized with the fluorescent label 2AB.
PMID: 31256372 [PubMed - in process]

Source: Mol Biol Cell - July 2, 2019 Category: Molecular Biology Authors: Grunow D, Blanchard V Tags: Methods Mol Biol Source Type: research