Giardia lamblia assemblages A and B isolated from symptomatic and asymptomatic persons in Hamadan, west of Iran

AbstractGiardia is a very abundant organism bringing about diarrhoea in human beings. The focus of this analysis was the detection ofGiardia lamblia assemblages in human stool specimens in Hamadan, west of Iran, as well as the association between obtained assemblages and clinical symptoms. Faecal samples of 4066 individuals admitted to  the medical and health care facilities in Hamadan were inspected microscopically for the existence ofGiardia cysts/trophozoites, and the clinical symptoms of the patients were recorded. The DNA of positive samples  was isolated from and the nucleotide sequences of both glutamate dehydrogenase (gdh) (n  = 15) and triose phosphate isomerase (tpi) (n  = 8) genes were analyzed. In direct microscopy, a total of sixty-four samples (1.6%), were considered as positive forG. lamblia cysts or trophozoites. The sequence analysis showed that 18 out of 23 sequenced isolates (78.2%) were assemblage A and 5 (21.7%) were assemblage B. Clinical symptoms were observed in 44.4% and 40% of patients with assemblages A and B, respectively. Overall, the predominant assemblage A detected in the tested samples along with bioinformatics analysis suggest a potential zoonotic transmission in the region of the study. Although advanced analyses are necessary to understand the foundation and path of the infection, it seems that more sanitary regulations regarding contact with livestock and pet animals are essential.
Source: Journal of Parasitic Diseases - Category: Parasitology Source Type: research

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Publication date: 1 March 2020Source: Materials Chemistry and Physics, Volume 243Author(s): Ahmed gamal El-ShamyAbstractIn this literature, magnesium peroxide (MgO2) nanorods with an average Ddiameter = 166 nm have been successfully synthesized via fast and simple microwave assisted hydrothermal reaction for photocatalysis and antibacterial applications, for the first time. The as synthesized MgO2 nanorods are characterized by various techniques like XRD, FTIR, TEM, FE-SEM and BET. The photocatalytic activity of MgO2 nanorods has been studied for methyl orange (MO) degradation, and it is found that MO dye ha...
Source: Materials Chemistry and Physics - Category: Materials Science Source Type: research
In this study, composite gels based on konjac and fish gelatin (FG) were prepared by alkali processing and thermal treatment subsequently. The influence of FG contents on the gel fraction, swelling behavior, biodegradation. was researched. Matrine was incorporated into the hydrogel and its effect on the hemocompatibility and antibacterial activity of gels were evaluated. According to the results, all gels presented at least 270% swelling degree and 72.7%∼81.5% equilibrium water content. Pure konjac hydrogel presented 83.9% gel fraction and 96.9% water evaporation rate, while those of the composite gels were 84.8%∼9...
Source: Food Hydrocolloids - Category: Food Science Source Type: research
CONCLUSION: Although the number of cases is small, the clinical and microbiological outcomes showed that most were successfully treated with non-carbapenem antibiotics, with low recurrence of new episodes of urinary tract infections. PMID: 31962058 [PubMed - as supplied by publisher]
Source: Acta Medica Portuguesa - Category: General Medicine Tags: Acta Med Port Source Type: research
CONCLUSION: Anterior segment optical coherence tomography can complement biomicroscopy, allowing for a better characterization of corneal involvement at presentation that can help in staging and providing useful prognostic information. PMID: 31962057 [PubMed - as supplied by publisher]
Source: Acta Medica Portuguesa - Category: General Medicine Tags: Acta Med Port Source Type: research
This study aims to identify the common risk factors and associated symptoms of menstrual pain which have been previously not caught the attention of researchers in Ghana.Methodology: The study was a descriptive cross-sectional study involving to two hundred female undergraduate students of the University of Cape Coast (UCC), Ghana. Data collected and analysed using standardized and acceptable statistical tools. Verbal multidimensional scoring system for assessment of dysmenorrhoea severity was used in this study to assess the severity of dysmenorrhoeaResults: More than half (57.3%) of the respondents having pain beginning ...
Source: African Health Sciences - Category: African Health Source Type: research
Conclusion: Due to general lack of awareness towards toxoplasmosis observed among both health workers and pregnant women in Temeke Municipality, we recommend health policy on maternal and child healthcare to address prenatal screening that is aimed at providing early diagnosis for any possible congenital toxoplasmosis as well as diseases that are currently screened in Tanzania such as HIV, syphilis and malaria. Integrating a One Health approach in educating medical professionals and the vulnerable population of pregnant women on the importance of congenital zoonoses will promote awareness and preventive practices towards t...
Source: African Health Sciences - Category: African Health Source Type: research
Introduction: Gram-negative bacteria are the major cause of urinary tract infections (UTI) in children. There is limited data on UTI systemic response as measured using C-reactive protein (CRP). Here, we report the association of CRP and UTI among children attending the Bugando Medical Centre, Mwanza, Tanzania.Methods: A cross-sectional study was conducted between May and July 2017. Urine and blood were collected and processed within an hour of collection. Data were analyzed using STATA version 13.Results: Of 250 enrolled children, 76(30.4%) had significant bacteriuria with 56(22.4%, 95%CI; 11.5-33.3) having gram-negative ...
Source: African Health Sciences - Category: African Health Source Type: research
a IM, Müller N, Robertson LJ Abstract Giardia duodenalis is one of the most important intestinal parasites globally, especially in children, and in Cuba is the leading cause of chronic paediatric diarrhoea in this population. G. duodenalis is composed of eight genetic groups (or assemblages), two of which (A and B) are apparently zoonotic, occurring in both humans and other animals. However, consensus on the most appropriate genotyping scheme for optimal characterization of G. duodenalis isolates is lacking. In this article we present the results of three descriptive observational studies conducted in Havana,...
Source: Experimental Parasitology - Category: Parasitology Authors: Tags: Exp Parasitol Source Type: research
Abstract Foodborne zoonotic pathogens are a serious public health issue and result in significant global economic losses. Despite their importance to public health, epidemiological data on foodborne diseases including giardiasis caused by the enteric parasite, Giardia duodenalis, are lacking. This parasite is estimated to cause ∼28.2 million cases of diarrhoea each year due to contamination of food, but very few foodborne outbreaks have been documented due to the limitations of current detection as well as surveillance methods. The current method for the recovery of Giardia cysts from food matrices using immun...
Source: International Journal for Parasitology - Category: Parasitology Authors: Tags: Int J Parasitol Source Type: research
Summary There appears to be no published information concerning the awareness and knowledge about diarrhoea caused by Cryptosporidium spp. or Giardia lamblia among US paediatricians and caregivers of young children. Two concurrent, separate surveys were conducted among paediatricians and caregivers (~1000 respondents in each survey) of children ages 1–12 years concerning their knowledge, perceptions and attitudes in the diagnosis and treatment of parasitic diarrhoea. Awareness of parasite‐induced diarrhoea was low for specific aspects among both paediatricians and caregivers. Educational efforts to improve awa...
Source: Zoonoses and Public Health - Category: Infectious Diseases Authors: Tags: Original Article Source Type: research
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