Immunostimulatory and antibacterial activity of Leuconostoc mesenteroides and its purified exopolysaccharide against extended-spectrum beta-lactamase producing Burkholderia cepacia

This study was aimed to extract, purified, and characterize the EPS from Leuconostoc mesenteroides subsp. Cremoris and to evaluate the immunostimulatory and antibacterial activities of EPS against extended-spectrum beta-lactamase (ESBL) producing Burkholderia cepacia strains. Nine EPSs producing L. mesenteroides subsp. cremoris strains were isolated from local dairy products and the isolated bacteria were identified by using API 50. Eight B. cepacia strains were isolated from different specimens in the hospitals of Medical city, Baghdad. Furthermore, genotypic and phenotypic detection of antibiotic resistance were determined including ESBL genes. EPS of L. mesenteroides subsp. Cremoris was extracted and purified by gel filtration chromatography. EPS physical and chemical analysis were performed to characterize it. Antibacterial and immunomodulatory effect of EPS were studied in vivo using mice and ELISA was used to determine the levels of IL-10 in the mice sera. The extracted EPS was found to have a maximum relative viscosity in water (3.51 dl/g) and maximum specific viscosity (2.93 dl/g), while the intrinsic viscosity recorded 1.41 dl/g. The chemical analysis of the extracted polysaccharide was found to contain the following components, carbohydrates, protein, uronic acids, hexosamines, acetyl groups, ketal linked pyruvate groups, phosphate groups, and sulfate groups, also show the following functional groups under infrared (IR) spectra (hydroxyl, alkanes, carbonyl, ca...
Source: Reviews in Medical Microbiology - Category: Microbiology Tags: BACTERIOLOGY Source Type: research