High-speed atomic force microscopy.

High-speed atomic force microscopy. Curr Opin Chem Biol. 2019 Jun 26;51:105-112 Authors: Ando T Abstract High-speed atomic force microscopy (HS-AFM) is a unique tool for molecular imaging. It can directly visualize protein molecules during their functional activity at high spatiotemporal resolution, without a marker being attached to the molecules. Molecular dynamics filmed with HS-AFM can provide mechanistic insights into the functional molecular processes that are hard to be attained with other approaches. In this mini review, I highlight some of recent relevant studies of proteins by HS-AFM imaging after brief descriptions of AFM and HS-AFM. PMID: 31254806 [PubMed - as supplied by publisher]
Source: Current Opinion in Chemical Biology - Category: Biochemistry Authors: Tags: Curr Opin Chem Biol Source Type: research

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Publication date: Available online 28 January 2020Source: Trends in GeneticsAuthor(s): Feras E. Machour, Nabieh AyoubDefective double-strand break (DSB) repair leads to genomic instabilities that may augment carcinogenesis. DSBs trigger transient transcriptional silencing in the vicinity of transcriptionally active genes through multilayered processes instigated by Ataxia telangiectasia mutated (ATM), DNA-dependent protein kinase (DNA-PK), and poly-(ADP-ribose) polymerase 1 (PARP1). Novel factors have been identified that ensure DSB-induced silencing via two distinct pathways: direct inhibition of RNA Polymerase II (Pol II...
Source: Trends in Genetics - Category: Genetics & Stem Cells Source Type: research
In this study, 10 nm CdO core-PEG stabilized NPs were synthesized, characterized and used for evaluation of CdO NPs’ cytotoxicity and genotoxicity. Release of cadmium ions (Cd+2) from the CdO NPs in cell culture medium, cellular uptake of the NPs, and the endotoxin content of the particles were measured prior to the toxicity assays. Cytotoxicity was evaluated using the MTS assay, ATP content detection assay, and LDH assay. Genotoxicity was assessed using the Ames test, Comet assay, micronucleus assay, and mouse lymphoma assay. The cytotoxicity of cadmium chloride (CdCl2) was also evaluated along with that of t...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - Category: Genetics & Stem Cells Source Type: research
Publication date: Available online 28 January 2020Source: Mutation Research/Genetic Toxicology and Environmental MutagenesisAuthor(s): Lisbeth E. Knudsen, David H. Phillips, Micheline Kirsch-VoldersAbstractEEMS and its successor Society EEMGS have provided a dynamic and successful platform to stimulate research and exchanges among the different actors involved in the protection of the environment and of human health from exposure to genome stressors. It includes basic, translational and applied research projects. This was possible due to the enthusiasm, creativity and support of scientists convinced of the importance of th...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - Category: Genetics & Stem Cells Source Type: research
Publication date: Available online 28 January 2020Source: Journal of Genetics and GenomicsAuthor(s): Leidy D. Caraballo Galva, Lun Cai, Yanxia Shao, Yukai HeAbstractLiver cancers, majority of which are primary hepatocellular carcinoma (HCC), continue to be on the rise in the world. Furthermore, due to the lack of effective treatments, liver cancer ranks the 4th most common cause of male cancer deaths. Novel therapies are urgently needed. Over last few years, immunotherapies, especially the checkpoint blockades and adoptive cell therapies of engineered T cells, have demonstrated a great potential for treating malignant tumo...
Source: Journal of Genetics and Genomics - Category: Genetics & Stem Cells Source Type: research
Publication date: Available online 27 January 2020Source: Genes &DiseasesAuthor(s): Fengji Liang, Yuan Quan, Andong Wu, Ying Chen, Ruifeng Xu, Yuexing Zhu, Jianghui XiongAbstractInsulin-resistance (IR) is one of the most important precursors of type 2 diabetes (T2D). Recent evidence suggests an association of depression with the onset of T2D. Accumulating evidence shows that depression and T2D share common biological origins, and DNA methylation examination might reveal the link between lifestyle, disease risk, and potential therapeutic targets for T2D. Here we hypothesize that integrative mining of IR and depression c...
Source: Genes and Diseases - Category: Genetics & Stem Cells Source Type: research
Publication date: Available online 27 January 2020Source: Genes &DiseasesAuthor(s): Weiyi Wang, Chao WeiAbstractHepatocellular carcinoma (HCC) is one of the most prevalent cancers globally. In contrast to the declining death rates observed for all other common cancers such as breast, lung, and prostate cancers, the death rates for HCC continue to increase by ∼2-3% per year because HCC is frequently diagnosed late and there is no curative therapy for an advanced HCC. The early diagnosis of HCC is truly a big challenge. Over the past years, the early diagnosis of HCC has relied on surveillance with ultrasonography (U...
Source: Genes and Diseases - Category: Genetics & Stem Cells Source Type: research
Publication date: Available online 11 January 2020Source: Biochimica et Biophysica Acta (BBA) - Gene Regulatory MechanismsAuthor(s): Maxence Lejars, Eliane HajnsdorfAbstractBacteria exhibit an amazing diversity of mechanisms controlling gene expression to both maintain essential functions and modulate accessory functions in response to environmental cues. Over the years, it has become clear that bacterial regulation of gene expression is still far from fully understood. This review focuses on antisense RNAs (asRNAs), a class of RNA regulators defined by their location in cis and their perfect complementarity with their tar...
Source: Biochimica et Biophysica Acta (BBA) Gene Regulatory Mechanisms - Category: Genetics & Stem Cells Source Type: research
New England Journal of Medicine, Ahead of Print.
Source: New England Journal of Medicine - Category: Internal Medicine Authors: Source Type: research
New England Journal of Medicine, Ahead of Print.
Source: New England Journal of Medicine - Category: Internal Medicine Authors: Source Type: research
Publication date: March 2020Source: International Journal of Industrial Ergonomics, Volume 76Author(s): Erman Çakıt, Andrzej Jan Olak, Waldemar Karwowski, Tadeusz Marek, Irena Hejduk, Redha TaiarAbstractThe main objective of this research was to apply an adaptive neuro-fuzzy inference system (ANFIS) approach aided by Partial Least Squares Structural Equation Modeling (PLS-SEM) to assess safety at work, defined as employee propensity to follow safety regulations, including safe work practices at the workplace. A survey with seven main components: 1) use of mobile technology, 2) tacit safety knowledge, 3) explicit sa...
Source: International Journal of Industrial Ergonomics - Category: Occupational Health Source Type: research
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