Comprehensive quantification of sesame allergens in processed food using liquid chromatography-tandem mass spectrometry

In this study, we developed a qualitative and absolute quantification method based on the LC-MS/MS-multiple reaction monitoring (MRM) system utilizing stable isotope-labeled internal standard (SIIS) peptides. We optimized extraction and trypsin digestion conditions and selected signature and absolute quantification (AQUA) peptides for seven sesame allergen proteins respectively. This quantitative method produced a linear relationship (R2 > 0.99) in a wide concentration range (0.4–2000 fmol/μL), and the overall coefficient of variation was <5% in multiple tests. Limits of detection (LOD) and Limits of quantification (LOQ) for the seven sesame allergens AQUA peptides were determined to range from 0.1 to 140.0 fmol/μL and 0.4–400 fmol/μL respectively. The results showed that the accuracy of 20, 50, 100 ppm incurred protein Ses i 2 were 90%, 92%, 94% in non-processed materia and 92%, 95%,102% in processed material respectively. Overall, this method allowed for rapid and simultaneous absolute quantification of seven sesame allergens. The newly developed LC-MS/MS assay will likely increase the safety of sesame processed foods and add diagnostic value to the sesame allergy testing.
Source: Food Control - Category: Food Science Source Type: research