Morphology-based classification of mycobacteria-infected macrophages with Convolutional Neural Network: Reveal EsxA-induced morphological changes indistinguishable by naked eyes

In this study, we use light microscopy and deep learning-based image analysis to classify the morphological changes of macrophages infected by Mycobacterium marinum (Mm), a surrogate model for Mtb. Macrophages were infected either with the mCherry-expressing Mm wild type strain (Mm(WT)), or a mutant strain with deletion of the esxA-esxB operon (Mm( ΔEsxA:B)).
Source: Translational Research - Category: Research Authors: Source Type: research