Feasible development of stable HEK293 clones by CRISPR/Cas9-mediated site-specific integration for biopharmaceuticals production.

CONCLUSIONS: CRISPR/Cas9 mediated site-specific integration is an efficient and reliable tool to establishment recombinant stable HEK293 cell lines for both academic and industrial applications.
PMID: 31236787 [PubMed - as supplied by publisher]

Source: Biotechnology Letters - June 23, 2019 Category: Biotechnology Authors: Yang H, Wang J, Zhao M, Zhu J, Zhang M, Wang Z, Gao Y, Zhu W, Lu H Tags: Biotechnol Lett Source Type: research