Crystal structure of (S)-3-O-geranylgeranylglyceryl phosphate synthase from Thermoplasma acidophilum in complex with the substrate sn-glycerol 1-phosphate

In this study, the crystal structure of the archaeal group II GGGPS from Thermoplasma acidophilum (TaGGGPS) was determined at 2.35   Å resolution. The structure of TaGGGPS showed that it has a TIM-barrel fold, the third helix of which is disordered ( α 3*), and that it forms a homodimer, although a pre-existing structure of an archaeal group II GGGPS (from Methanothermobacter thermautotrophicus) showed a hexameric form. The structure of TaGGGPS showed the precise G1P-recognition mechanism of an archaeal group II GGGPS. The structure of TaGGGPS and molecular-dynamics simulation analysis showed fluctuation of the β 2 – α 2, α 3* and α 5a regions, which is predicted to be important for substrate uptake and/or product release by TaGGGPS.

Source: Acta Crystallographica Section F - June 25, 2019 Category: Biochemistry Authors: Nemoto, N. Miyazono, K. Tanokura, M. Yamagishi, A. Tags: geranylgeranylglyceryl phosphate synthase TIM barrel archaea prenyltransferases ether lipid biosynthesis research communications Source Type: research