Expression of bitter taste receptors and solitary chemosensory cell markers in the human sinonasal cavity.

Expression of bitter taste receptors and solitary chemosensory cell markers in the human sinonasal cavity. Chem Senses. 2019 Jun 20;: Authors: Chen J, Larson ED, Anderson CB, Agarwal P, Frank DN, Kinnamon SC, Ramakrishnan VR Abstract Some bitter taste receptors, (TAS2R gene products) are expressed in the human sinonasal cavity and may function to detect airborne irritants. The expression of all 25 human bitter taste receptors and their location within the upper airway is not yet clear. The aim of this study is to characterize the presence and distribution of TAS2R transcripts and solitary chemosensory cells (SCCs) in different locations of the human sinonasal cavity. Biopsies were obtained from human subjects at up to four different sinonasal anatomic sites. PCR, microarray, and qRT-PCR were used to examine gene transcript expression. The 25 human bitter taste receptors as well as the sweet/umami receptor subunit, TAS1R3, and canonical taste signaling effectors are expressed in sinonasal tissue. All 25 human bitter taste receptors are expressed in the human upper airway, and expression of these gene products was higher in the ethmoid sinus than nasal cavity locations. Fluorescent in situ hybridization demonstrates that epithelial TRPM5 and TAS2R38 are expressed in a rare cell population compared to multiciliated cells, and at times, consistent with SCC morphology. Secondary analysis of published human sinus single cell RNAseq data di...
Source: Chemical Senses - Category: Biochemistry Authors: Tags: Chem Senses Source Type: research