Induction of chemokine CCL3 by NF-κB reduces methylmercury toxicity in C17.2 mouse neural stem cells

Publication date: Available online 24 June 2019Source: Environmental Toxicology and PharmacologyAuthor(s): Tsutomu Takahashi, Min-Seok Kim, Miyuki Iwai-Shimada, Takayuki Hoshi, Masatake Fujimura, Takashi Toyama, Yasuyuki Fujiwara, Akira Naganuma, Gi-Wook HwangAbstractMethylmercury is an environmental pollutant that shows selective toxicity to the central nervous system. We previously reported that brain-specific expression of chemokine CCL3 increases in mice administered methylmercury. However, the relationship between CCL3 and methylmercury toxicity has not been elucidated. Here, we confirmed that induction of CCL3 expression occurs before pathological change by methylmercury treatment was observed in the mouse brain. This induction was also observed in C17.2 mouse neural stem cells before methylmercury-induced cytotoxicity. In addition, cells in which CCL3 was knocked-down showed higher methylmercury sensitivity than did control cells. Moreover, activation of transcription factor NF-κB was observed following methylmercury treatment, and methylmercury-mediated induction of CCL3 expression was partially suppressed by knockdown of p65, an NF-κB subunit. Our results suggest that NF-κB plays a role in the induction of methylmercury-mediated CCL3 expression and that this action may be a cellular response to methylmercury toxicity.
Source: Environmental Toxicology and Pharmacology - Category: Environmental Health Source Type: research

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Mesenchymal stem cells (MSCs) possess anti-inflammatory properties and tissue differentiation potential. We prepared a rat shoulder arthritis model by injecting monoiodoacetate (MIA) into the shoulder and investigated the intraarticular administration of MSCs from the aspects of the cartilage protective effect associated with the cell ’s anti-inflammatory activity and inhibition of the central sensitization of pain. When MIA was administered in this model, anti-calcitonin gene related peptide (CGRP) was expressed in the shoulder joint and C5 spinal dorsal horn.
Source: Journal of Shoulder and Elbow Surgery - Category: Orthopaedics Authors: Source Type: research
Publication date: January 2020Source: Materials Science and Engineering: C, Volume 106Author(s): Shaohua Wu, Rong Zhou, Fang Zhou, Philipp N. Streubel, Shaojuan Chen, Bin DuanAbstractMicrofiber yarns (MY) have been widely employed to construct tendon tissue grafts. However, suboptimal ultrastructure and inappropriate environments for cell interactions limit their clinical application. Herein, we designed a modified electrospinning device to coat poly(lactic-co-glycolic acid) PLGA nanofibers onto polylactic acid (PLA) MY to generate PLGA/PLA hybrid yarns (HY), which had a well-aligned nanofibrous structure, resembling the u...
Source: Materials Science and Engineering: C - Category: Materials Science Source Type: research
Publication date: January 2020Source: Materials Science and Engineering: C, Volume 106Author(s): Ariel C. de Oliveira, Roberta M. Sabino, Paulo R. Souza, Edvani C. Muniz, Ketul C. Popat, Matt J. Kipper, Rafael S. Zola, Alessandro F. MartinsAbstractHere, we have demonstrated the production and characterization of hydrogel scaffolds based on chitosan/gellan gum (CS/GG) assemblies, without any covalent and metallic crosslinking agents, conventionally used to yield non-soluble polysaccharide-based materials. The polyelectrolyte complexes (physical hydrogels called as PECs) are characterized by Fourier-transform infrared spectr...
Source: Materials Science and Engineering: C - Category: Materials Science Source Type: research
We present an alternative method for studying these issues using the axonal transport of Herpes simplex virus in mature retinal neurons in vivo. Using genetically identical mice and carefully controlling the delivery of virus, an investigator can obtain insight into the transport of virus to and from the neuron cell body within the cellular environment of an intact, mature animal. This allows confirmation and extension of results seen in vitro. PMID: 31617195 [PubMed - in process]
Source: Herpes - Category: Infectious Diseases Authors: Tags: Methods Mol Biol Source Type: research
AbstractBone marrow failure has many different etiologies, including genetic defects which manifest with specific syndromes, as well as acquired conditions as a result of insults to the bone marrow leading to aplasia. The clinical picture is varied and clues for the underlying cause may or may not be evident at the time of presentation, frequently leading to a complex workup with a battery of tests often done to rule out genetic defects. The treatment approach for bone marrow failure is very dependent on the underlying cause, which makes it all the more critical to have an accurate diagnosis. First line management essentia...
Source: Indian Journal of Pediatrics - Category: Pediatrics Source Type: research
Publication date: Available online 16 October 2019Source: Chemico-Biological InteractionsAuthor(s): Yasmeen M. Attia, Dina M. El-Kersh, Reham A. Ammar, Aliaa Adel, Aya Khalil, Hoda Walid, Kirullos Eskander, Mohamed Hamdy, Nada Reda, Nour Elhoda Mohsen, Ghada M. Al-Toukhy, Mohamed Tarek Mansour, Mohamed M. ElmazarAbstractTreatment of breast cancer by paclitaxel (PAX) often encounters therapeutic failure most likely caused by innate/acquired resistance. Cancer stem cells (CSCs) and multidrug resistance complex (MDR-1 or P-glycoprotein) overexpression are main mechanisms implicated in chemoresistance. Increased aldehyde dehro...
Source: Chemico Biological Interactions - Category: Biochemistry Source Type: research
THURSDAY, Oct. 17, 2019 -- Different racial/ethnic groups with multiple myeloma (MM) have significant variations in novel therapy and autologous stem cell transplant (ASCT) use, according to a study published online Oct. 17 in Blood...
Source: Drugs.com - Pharma News - Category: Pharmaceuticals Source Type: news
Condition:   Knee Osteoarthritis Interventions:   Biological: Low Dose of Mesenchymal stem cell;   Biological: High Dose of Mesenchymal stem cell;   Drug: Sodium Hyaluronate Sponsor:   CAR-T (Shanghai) Biotechnology Co., Ltd. Recruiting
Source: ClinicalTrials.gov - Category: Research Source Type: clinical trials
Extended pluripotent stem cells from mice can self-organize to form blastocyst-like structures called blastoids that enable a closer look at early mammalian development.
Source: The Scientist - Category: Science Tags: News & Opinion Source Type: news
ConclusionThis study reiterates the importance of individual profiling of gut microbiota in HSCT patients.
Source: Medical Journal Armed Forces India - Category: General Medicine Source Type: research
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