GSE133205 Gene expression in gastric Mist1+ cells.

Contributors : Masahiro Hata ; Hiroto Kinoshita ; Yoku Hayakawa ; Mitsuru Konishi ; Mayo Tsuboi ; Yukiko Oya ; Hayato Nakagawa ; Hiroaki Fujiwara ; Samuel Asfaha ; Daniel L Worthley ; Yuki Muranishi ; Takahisa Furukawa ; Shunsuke Kon ; Hiroyuki Tomita ; Timothy C Wang ; Kazuhiko KoikeSeries Type : Expression profiling by arrayOrganism : Mus musculusMist1+ cells and parietal cells in mouse stomach were separatedly sorted, and RNAs were isolated.Mist1 (also known as Bhlha15) is expressed in gastric chief cells and gastric stem cells in mice. However, more specific genes for each population needs to be identified to better understand the precise biology in these cell populations. In order to address cell specific gene signature, we separately sorted Mist1+ gastric chief cells and Mist1+ gastric stem cells by FACS, and performed microarray analysis. Mist1+ gastric chief cells were sorted by using Mist1-CreERT; R26-TdTomato mouse stomach, immediately after tamoxifen administration. Mist1+ gastric stem cells were sorted by chief cell-ablated Mist1-CreERT; R26-TdTomato mouse stomach, combining with Lgr5-DTR mice. Lgr5-expressing chief cells were ablated by giving DT into these mice. As a control, acid-secreting gastric parietal cell samples were used.Mice were treated with or without Lgr5-DT ablation before sorting.
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by array Mus musculus Source Type: research