Inhibitory Effect of Volatiles Emitted From Alcaligenes faecalis N1-4 on Aspergillus flavus and Aflatoxins in Storage

Controlling aflatoxigenic Aspergillus flavus (Af) and aflatoxins in grains and food during storage are great challenge to humans worldwide. Alcaligenes faecalis N1-4 isolated from tea rhizosphere soil can produce abundant antifungal volatiles, and greatly inhibited the growth of Af in un-contacted face-to-face dual culture tests. Gas chromatography tandem mass spectrometry revealed that dimethyl disulfide (DMDS) and methyl isovalerate (MI) were two abundant compounds in the volatile profiles of N1-4. DMDS with the highest relative abundance (69.90%, to the total peak area) in N1-4 volatiles eventually prevented the conidia germination and mycelia growth of Af at 100 µL/L respectively. The concentration for MI against Af is 200 µL/L. Additionally, Real‑time Quantitative PCR analysis proved that the expression of 11 important genes in aflatoxin biosynthesis pathway were significantly reduced by these volatiles. And the Af disease and aflatoxins contamination in groundnut, maize, rice and soybean of high water activity were also completely inhibited by volatiles in storage. Scanning electron microscope further proved that Af conidia inoculated on peanuts surface were severely damage by volatiles from N1-4, and can not germinate to hyphae and conidiophore. Furthermore, strain N1-4 showed broad and effective antifungal activity to other 6 important plant pathogens including Fusarium graminearum, F. equiseti, Alternaria alternate, Botrytis cinerea, Aspergillus niger a...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research

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Abstract The cellulase production by filamentous fungi Aspergillus fumigatus JCM 10253 was carried out using agro-industrial waste ragi husk as a substrate in the microbial fermentation. The effect of the process parameters such as temperature, substrate concentration, pH, and incubation process time and their interdependence was studied using response surface methodology. The optimum cellulase activities were obtained at 50 °C under the conditions with 1-2% of substrate concentration at pH 2-4 for the incubation period of 7-8 days. The maximum carboxymethyl cellulase (CMCase) and β-glucosidase ...
Source: Bioprocess and Biosystems Engineering - Category: Biomedical Engineering Authors: Tags: Bioprocess Biosyst Eng Source Type: research
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Source: The Aspergillus Website - updates - Category: Respiratory Medicine Authors: Source Type: news
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Source: Bioprocess and Biosystems Engineering - Category: Biomedical Engineering Authors: Tags: Bioprocess Biosyst Eng Source Type: research
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Source: Polish Journal of Microbiology - Category: Microbiology Tags: Pol J Microbiol Source Type: research
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Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
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Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
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