Organometallic Cerium Oxide Nanostructures acted as Nanofertilizer during Callogenesis and Organogenesis of Recalcitrant Plant Berberis lycium Royle

We report organometallic cerium oxide nanostructures (OM-CeO2-NS) mediated in vitro callus induction and direct shoot regeneration of medicinally important and recalcitrant plant (Berberis lycium Royle). To achieve greater biocompatibility, Hypericum scabrum flower extract was used during the green synthesis process. Ultra-performance Liquid Chromatography-Diode Array Detector (UPLC-DAD) coupled with Mass spectrometry (MS) was employed to detect the potential secondary metabolites in H. scabrum flower extract which acted as reducing as well as the surface stabilizing agents for tailoring the morphology of OM-CeO2-NS. High resolution Transmission electron microscopy (Hr-TEM) and Atomic Force Microscopy (AFM) examination of the prepared OM-CeO2-NS revealed homogenous morphology with an average size of 38 ± 5 nm. Moreover, Fourier transform infrared (FT-IR) spectroscopy examination and X-ray diffraction (XRD) technique also confirm organometallic chemistry of prepared CeO2-NS. Interestingly, nutritive behaviour of OM-CeO2-NS was observed in B. lycium tissue culturing primarily due to the extremely small size and organometallic nature. The results reveal that callus induction is enhanced to 90% with low concentration of OM-CeO2-NS (4 mg/L) and direct shoot regeneration is improved to 79% with increased concentration of 20 mg/L. The current study concludes that OM-CeO2-NS can be used as nanofertilizer in tissue culturing of the recalcitrant plant species.Graphical abstr...
Source: Materials Today Communications - Category: Materials Science Source Type: research