Mycoplasma hyopneumoniae evades phagocytic uptake by porcine alveolar macrophages in vitro
AbstractMycoplasma hyopneumoniae, the agent of porcine enzootic pneumonia (EP), is able to persist in the lung tissue and evade destruction by the host for several weeks. To understand the mechanism of pathogen survival, phagocytic uptake ofM. hyopneumoniae by primary porcine alveolar macrophages was investigated. Intracellular location and survival of the pathogen were explored using gentamicin survival assays, flow cytometry and confocal microscopy ofM. hyopneumoniae 232 labelled with green fluorescent protein (GFP). Following 1 h and 16 h of co-incubation, few viableM. hyopneumoniae were recovered from inside macrophages. Flow cytometric analysis of macrophages incubated withM. hyopneumoniae expressing GFP indicated that the mycoplasmas became associated with macrophages, but were shown to be extracellular when actin-dependent phagocytosis was blocked with cytochalasin D. Confocal microscopy detected GFP-labelledM. hyopneumoniae inside macrophages and the numbers increased modestly with time of incubation. Neither the addition of porcine serum complement or convalescent serum from EP-recovered pigs was able to enhance engulfment ofM. hyopneumoniae. This investigation suggests thatM. hyopneumoniae evades significant uptake by porcine alveolar macrophages and this may be a mechanism of immune escape byM. hyopneumoniae in the porcine respiratory tract.
A recap of Tuesday’s top stories: Pneumonia number one killer of children; Australia’s wildfires rage on; Ebola vaccine gets green light; new child labour and trafficking study; Malnutrition soars in Latin America and Caribbean; Intellectual Property chief encourages innovation.
In conclusion, we emphasize that it is of importance to work closely with the hospital administration to take measures and that necessary assistance is provided. PMID: 31709934 [PubMed - in process]
CONCLUSION: This study identified prior antibiotic exposure, recent surgery and the use of invasive procedures as significant risk factors for colonization or infection with CRE. Also, the need for public awareness, continuing education for healthcare professionals, optimum use of invasive devices, enhanced surveillance, and antimicrobial stewardship are highlighted here which can limit CRE transmission in healthcare facilities. PMID: 31707406 [PubMed - in process]
Pneumonia, an entirely preventable disease, kills more children than any other illness in the world, one child every 39 seconds. But although that statistic is well known, funding to improve survival rates continues to come up short, the UN and partners warned on Tuesday, World Pneumonia Day.
By Alice Rwema Iribagiza, Communications Officer, Ingobyi Nurse Esther at work. Photo credit: IntraHealth International.November 12, 2019Esther Mukahabiyambere is one of 326 district-based mentors trained on the Integrated Management of Childhood Illness (IMCI) approach. Through the USAID-fundedIngobyi Activity, health workers are using IMCI in Rwanda to prevent and treat pneumonia, diarrhea, malnutrition, and malaria. The project equips health care providers with clinical knowledge, skills, and attitudes to improve the quality of care, helping the government of Rwanda reduce child mortality, particularly the managemen...
[Daily Trust] The death of 162,000 Nigerian children, of pneumonia in 2018, is the highest of the estimated total of 802,000 pneumonia deaths globally, the Save the Children said this in Abuja.
[This Day] Abuja -A leading child rights organisation, Save the Children, has revealed that pneumonia claimed the lives of 162,000 children below the age of five in Nigeria in 2018.
[Vanguard] A new report by the United Nations Children Fund (UNICEF) shows Nigeria recorded the highest global deaths from pneumonia at 162,000 last year.
(Murdoch Childrens Research Institute) A new study has found severe pneumonia decreases by 35 per cent in children who receive a vaccine against a pneumonia-causing bacteria.
Emergency admissions have risen more than 50 per cent over the last decade, with 56,000 British children hospitalised with the condition last year.