Mycoplasma hyopneumoniae evades phagocytic uptake by porcine alveolar macrophages in vitro

AbstractMycoplasma hyopneumoniae, the agent of porcine enzootic pneumonia (EP), is able to persist in the lung tissue and evade destruction by the host for several weeks. To understand the mechanism of pathogen survival, phagocytic uptake ofM. hyopneumoniae by primary porcine alveolar macrophages was investigated. Intracellular location and survival of the pathogen were explored using gentamicin survival assays, flow cytometry and confocal microscopy ofM. hyopneumoniae 232 labelled with green fluorescent protein (GFP). Following 1  h and 16 h of co-incubation, few viableM. hyopneumoniae were recovered from inside macrophages. Flow cytometric analysis of macrophages incubated withM. hyopneumoniae expressing GFP indicated that the mycoplasmas became associated with macrophages, but were shown to be extracellular when actin-dependent phagocytosis was blocked with cytochalasin D. Confocal microscopy detected GFP-labelledM. hyopneumoniae inside macrophages and the numbers increased modestly with time of incubation. Neither the addition of porcine serum complement or convalescent serum from EP-recovered pigs was able to enhance engulfment ofM. hyopneumoniae. This investigation suggests thatM. hyopneumoniae evades significant uptake by porcine alveolar macrophages and this may be a mechanism of immune escape byM. hyopneumoniae in the porcine respiratory tract.
Source: Veterinary Research - Category: Veterinary Research Source Type: research

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Source: UN News Centre - Health, Poverty, Food Security - Category: International Medicine & Public Health Source Type: news
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Source: AllAfrica News: Health and Medicine - Category: African Health Source Type: news
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