Polysaccharide extracted from Atractylodes macrocephala Koidz (PAMK) induce apoptosis in transplanted H22 cells in mice.

Polysaccharide extracted from Atractylodes macrocephala Koidz (PAMK) induce apoptosis in transplanted H22 cells in mice. Int J Biol Macromol. 2019 Jun 18;: Authors: Feng YY, Ji HY, Dong XD, Yu J, Liu AJ Abstract Polysaccharide of Atractylodes macrocephala Koidz (PAMK) was extracted by alcohol sedimenting ranging from 60% to 90% and purified by ultrafiltration membrane. The high-performance gel permeation chromatography (HPGPC), Fourier-transform infrared spectroscopy (FT-IR), gas chromatography (GC) and Nuclear magnetic resonance (NMR) revealed that PAMK was a 4.1KDa neutral heteropolysaccharide composed of galactose, arabinose and glucose with α-configuration (molar ratio, 1: 1.5: 5). Results of determination of chemical components suggested that PAMK contained 96.47% of polysaccharide and little protein, nucleic acid and uronic acid. Antitumor experiments in vivo could be concluded that PAMK had a significantly cytotoxic and anti-tumor effect by blocking tumor cells in S phase. And not only that, PAMK could protect immune organ efficiently, comparing with cyclophosphamide. The research provided a potential antineoplastic drug component for tumor treatment. PMID: 31226375 [PubMed - as supplied by publisher]
Source: International Journal of Biological Macromolecules - Category: Biochemistry Authors: Tags: Int J Biol Macromol Source Type: research