MicroRNA-221 promotes cisplatin resistance in osteosarcoma cells by targeting PPP2R2A.

MicroRNA-221 promotes cisplatin resistance in osteosarcoma cells by targeting PPP2R2A. Biosci Rep. 2019 Jun 20;: Authors: Yu WC, Chen HH, Qu YY, Xu CW, Yang C, Liu Y Abstract Osteosarcoma (OS), the most common malignant bone tumor, is the main cause of cancer-related death in children and young adults. Despite the combination of surgery and multi-agent chemotherapy, patients with OS who develop resistance to chemotherapy or experience recurrence have a dismal prognosis. MicroRNAs (miRNAs) are a class of small noncoding RNAs that repress their targets by binding to the 3'-UTR and/or coding sequences, leading to the inhibition of gene expression. miR-221 is found to be upregulated in tumors when compared to their matched normal osteoblast tissues.   We also observed significant miR-221 upregulation in the OS cell lines, MG-63, SaoS-2, and U2OS, when compared to the normal osteoblast cell line, HOb.   Overexpression of miR-221 promoted OS cell invasion, migration, proliferation, and cisplatin resistance.   MG-63 and SaoS-2 cells transfected with miR-221 mimics were more resistant to cisplatin. The IC50 of MG-63 cells transfected with control mimics was 1.24 μM. However, the IC50 of MG-63 cells overexpressing miR-221 increased to 7.65 μM. Similar results were found in SaoS-2 cells, where the IC50 for cisplatin increased from 3.65 μM to 8.73 μM. Thus, we report that miR-221 directly targets PPP2R2A in OS by binding to the 3'-UTR...
Source: Bioscience Reports - Category: Biomedical Science Authors: Tags: Biosci Rep Source Type: research