Non-targeted metabolomics reveals diagnostic biomarker in the tongue coating of patients with chronic gastritis

Publication date: Available online 20 June 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Xiyan Mu, Chuanyuan Ji, Qi Wang, Kun Liu, Xinyu Hao, Guanhua Zhang, Xiaowei Shi, Yuqian Zhang, Frank J. Gonzalez, Qiao Wang, Yangang WangAbstractAnalysis of the properties of the tongue has been used in traditional Chinese medicine for disease diagnosis. Notably, tongue analysis, which is non-invasive and convenient compared with gastroscopy and pathological examination, can be used to assess chronic gastritis (CG). In order to find potential diagnostic biomarkers and study the metabolic mechanisms of the endogenous small molecules in the tongue coating related to CG, a non-targeted metabolomic analysis method was developed using ultra high performance liquid chromatography combined with quadrupole time-of-flight mass spectrometry (UHPLC-Q/TOF-MS). It was performed using two different columns in positive and negative ion scanning modes separately. The stability of the samples was evaluated and the age and gender factors of the subjects were excluded to ensure the reliability of the data in this study. Finally, under the four analysis models, 130, 229, 113 and 92 differential compounds were found using multivariate statistical methods respectively. 37 potential biomarkers were putatively identified after removing the duplicate compounds and five potential diagnostic biomarkers were putatively identified by receiver operating characteristic (ROC) curve analysis, in...
Source: Journal of Pharmaceutical and Biomedical Analysis - Category: Drugs & Pharmacology Source Type: research