The Adh1 gene of the fungus Metarhizium anisopliae is expressed during insect colonization and required for full virulence

Publication date: Available online 5 December 2014 Source:Microbiological Research Author(s): Olga Alicia Callejas-Negrete , Juan Carlos Torres-Guzmán , Israel Enrique Padilla Guerrero , Ulises Esquivel-Naranjo , Maria Fernanda Padilla Ballesteros , Adriana García Tapia , Augusto Schrank , Eduardo Salazar-Solís , Félix Gutiérrez-Corona , Gloria Angélica González-Hernández Zymography of alcohol dehydrogenase (ADH) activity in the entomopathogenic fungus Metarhizium anisopliae grown under various conditions revealed that micro-aerobic growth was associated with increased ADH activity. The major ADH protein, AdhIp, was purified to homogeneity by affinity chromatography and has an estimated molecular weight of 41kDa and an isoelectric point (pI) of 6.4. Peptide mass fingerprint analysis allowed the identification and cloning of the gene that encodes this protein, Adh1, as annotated in the M. anisopliae genome database. AdhIp is related to the medium chain dehydrogenase/reductase (MDR)/zinc-dependent alcohol dehydrogenase-like family and contains conserved ADH sequence motifs, such as the zinc-containing ADH signature, the FAD/NAD binding domain and amino acid residues that are conserved in most microbial ADHs. Semi-quantitative RT-PCR analysis revealed that Adh1 gene expression occurs at low levels during early Plutella xylostella infection and that the Adh1 gene was primarily expressed at larval death and as mycelia emerge from the insect cuticle before con...
Source: Microbiological Research - Category: Infectious Diseases Source Type: research