Application of Deep Mutational Scanning in Hepatitis C Virus.

Application of Deep Mutational Scanning in Hepatitis C Virus.
Methods Mol Biol. 2019;1911:183-190
Authors: Wu NC, Qi H
Abstract
Mutagenesis is one of the key techniques in virus research. The recent development of deep mutational scanning allows the assessment of replication fitness effects of a large number of viral mutants in a high-throughput manner. Here, we describe a protocol for studying hepatitis C virus (HCV) using deep mutational scanning, which includes the methodologies for mutant library construction, passaging, sequencing, and data analysis.
PMID: 30593626 [PubMed - indexed for MEDLINE]

Source: Mol Biol Cell - June 20, 2019 Category: Molecular Biology Authors: Wu NC, Qi H Tags: Methods Mol Biol Source Type: research