Cell and tissue response to nanotextured Ti6Al4V and Zr implants using high-speed femtosecond laser-induced periodic surface structures

Publication date: Available online 19 June 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Iaroslav Gnilitskyi, Maksym Pogorielov, Roman Viter, Ana Maria Ferraria, Ana Patrícia Carapeto, Olexandr Oleshko, Leonardo Orazi, Oleg MishchenkoAbstractIn this paper, the effect of femtosecond laser nanotexturing of surfaces of Ti6Al4V and Zr implants on their biological compatibility is presented and discussed. Highly regular and homogeneous nanostructures with sub-micrometer period were imprinted on implant surfaces. Surfaces were morphologically and chemically investigated by SEM and XPS. HDFa cell lines were used for toxicity and cell viability tests, and subcutaneous implantation was applied to characterize tissue response. HDFa proliferation and in vivo experiments evidenced the strong influence of the surface topography compared to the effect of the surface elemental composition (metal or alloy). The effect of protein adsorption from blood plasma on cell proliferation is also discussed.Graphical AbstractFemtosecond nanotexturing (HR-LIPSS) used for surface modification of Ti6Al4V and Zr implants. Modified and non-modified materials were tested on in-vitro (HDFA cells) and in-vivo (rat) models. Cell proliferation and in-vivo results shown the strong dependence of biological response on surface topography and degree of oxidation of the surface, and suggested a predominant role of the surface topography, but also some influence of surface composition on th...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - Category: Nanotechnology Source Type: research