Dose-response relationships between copper and its biocompatibility/antibacterial activities

This study aimed to explore the dose-response relationships of Cu2+ and its biological effects in vitro. Rat bone marrow mesenchymal stem cell (rBMSCs), mouse osteoblastic cell line (MC3T3-E1), and human umbilical vein endothelial cells (HUVECs) were used to evaluate cellular behaviors. Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli) were used to evaluate bacterial behaviors.RESULTSResults showed that the HUVECs exhibited significantly higher tolerance to copper ions than MC3T3-E1 and rBMSCs. The IC50 values of copper for HUVECs, MC3T3-E1 and HUVECs were approximated to 327.9 μM, 134.6 μM, and 0.7 μM, respectively. Besides, the threshold concentration of copper for effective inhibition against bacteria growth is 37 μM. When the concentration exceeded the threshold value, antibacterial activity could increase dramatically.CONCLUTIONSThese results altogether establish a technological foundation for the application of copper-doped biomaterials in bone growth and remodeling.
Source: Journal of Trace Elements in Medicine and Biology - Category: Biochemistry Source Type: research