Supramolecular Off-On-Off Fluorescent Biosensor for Total Free Thyroid Hormones Detection Based on their Differential Binding with Cucurbit[7]uril to Fluorescent Perylene Derivative

Publication date: Available online 19 June 2019Source: Journal of Photochemistry and Photobiology A: ChemistryAuthor(s): Hamdy S. El-Sheshtawy, Ahmed El-Refaey, Mohamed E. El-KhoulyAbstractA novel host-guest biosensor composed of perylene dye derivatives and cucurbit[7]uril [CB7] is established for the determination of total thyroid hormones (triiodothyronine (T3) and thyroxine (T4)). The sensor was based on the competitive binding of the hormones (T3 and T4) with cucurbit[7]uril [CB7] to the fluorescent perylene derivative, N,N′-di(2-(trimethylammoniumiodide)ethylene) perylenediimide (TAIPDI). The binding constants between T3 and T4 with TAIPDI were determined to be 3.34 × 104 and 1.7 × 105 M–1, respectively, while the binding between TAIPDI and CB7 was found to be 1.48 × 103 M-1. The assembled biosensor showed different signal between T3 and T4 in the range of 0-106 and 0-25 μM and detection limit of 16.1 and 5.66 μM, respectively. The proposed sensor not only differentiates between the T3 and T4 hormones, but also accurately determines the total thyroid hormones, which is crucial for the hyperthyroidism clinical diagnosis. This strategy based on the use of fluorescent probe combined with macrocycle host is economical and tackle tedious and longtime procedures for similar methods.Graphical Abstract
Source: Journal of Photochemistry and Photobiology A: Chemistry - Category: Chemistry Source Type: research