GSE132903 Transcriptome changes in the Alzheimer's middle temporal gyrus: importance of RNA metabolism and mitochondria-associated membrane (MAM) genes

Contributors : Ignazio S Piras ; Jonida Krate ; Elaine Delvaux ; Jennifer Nolz ; Diego F Mastroeni ; Antonio M Persico ; Wayne M Jepsen ; Thomas G Beach ; Matthew J Huentelman ; Paul D ColemanSeries Type : Expression profiling by arrayOrganism : Homo sapiensWe used Illumina Human HT-12 v4 arrays to compare RNA expression of middle temporal gyrus (MTG; BA21) in Alzheimer ’s Disease (AD = 97) and non-demented controls (ND = 98). A total of 938 transcripts were highly differentially expressed (adj p< 0.01; log2 Fold Change (FC) ≥ |0.500|, with 411 overexpressed and 527 underexpressed in AD. Our results correlated with expression profiling in neurons from AD and ND obtained by Laser Capture Microscopy in MTG from an independent dataset (log2 FC correlation: r = 0.504; p = 2.2e-16). Additionally selected effects were va lidated by qPCR. ANOVA analysis yielded no difference between genders in response to AD, but some gender specific genes were detected (e.g: IL8 and AGRN in males, and HSPH1 and GRM1 in females). Several transcripts were associated with Braak Staging (e.g AEBP1 and DNALI1), ante-mortem MMSE (e.g. AE BP1 and GFAP) and Tangle density (eg. RNU1G2, and DNALI1). At the pathway level we detected enrichment of Synaptic Vesicle Processes and GABAergic transmission genes. Finally, applying the Weighted Correlation Network Analysis (WGCNA) we identified 4 expression modules enriched for neuronal and syna ptic genes, mitochondria-associated membrane (MAM), chemical...
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by array Homo sapiens Source Type: research